Method for evaluating and comparing immunorepertoires
Disclosed is a method for amplifying RNA and/or DNA from immune cell populations and using the amplified products to produce an immune response profile and evaluate the possible correlation between a normal or abnormal immune response and the development of a disease such as an autoimmune disease, cancer, diabetes, or heart disease.
1. A method comprising:
(a) selecting at least 2 V gene segment primers;
(b) selecting at least 2 J gene segment primers;
(c) combining the V gene segment and J gene segment primers with genomic DNA comprising rearranged nucleic acid molecules of a TCR region from lymphocytes obtained from a human or animal subject;
(d) amplifying, in one reaction, said rearranged nucleic acid molecules from said sample using said V gene segment and J gene segment primers in a first amplification reaction, thereby producing non-identical amplicons sufficient to evaluate an immune response, the non-identical amplicons representing a diversity of TCR genes;
wherein each of said at least 2 V gene segment primers is capable of annealing to V gene segment sequence and amplifying V gene segment sequence, and each of said at least 2 J gene segment primers is capable of annealing to J gene segment sequence and amplifying J gene segment sequence,
wherein each of said V gene segment primers comprises a first sequence and a second sequence, wherein said first sequence is complementary to a portion of at least one V gene segment, wherein said second sequence contains a binding site for a common primer, and
wherein each of said J gene segment primers comprises a first sequence and a second sequence, wherein said first sequence is complementary to a portion of a J gene segment, wherein said second sequence contains a binding site for a common primer;
(e) rescuing the amplicons from the first amplification reaction by dilution of the amplicons and primers from the first amplification reaction; and
(f) amplifying, in one reaction, the amplicons from the first amplification reaction in a second amplification reaction using at least one common primer which binds to the at least one common primer binding site and preserves the relative ratios of each amplicon from the first amplification reaction;
wherein diluted primers from the first amplification reaction are present during the second amplification reaction.
2. The method of claim 1 , wherein said first sequence of each V gene segment primer comprises a sequence that is complementary to a V Beta gene segment.
3. The method of claim 1 , further comprising sequencing the amplicons.
4. The method of claim 3 , wherein sequencing utilizes a set of primers that are complementary to a defined region within the amplicons.
5. The method of claim 4 , further comprising quantifying non-identical DNA sequences representing T-cell receptor Beta rearrangements among the amplicons.
6. The method of claim 5 , wherein the method allows amplification of the entire immunorepertoire of a human.