APPARATUS, SYSTEM, AND METHOD FOR COLLECTING A TARGET MATERIAL
This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a processing vessel, such as an Eppendorf tube, a syringe or a test tube, and a collector. The collector is sized and shaped to fit into a primary vessel, such as a test tube. The collector funnels the target material from the suspension through a cannula and into the processing vessel. The collector includes a funnel at a second end in fluid communication with the cannula. The cannula extends into a chamber at a first end of the collector that holds the processing vessel. In one implementation, the processing vessel is empty. In another implementation, the processing vessel includes at least one displacement fluid. In yet another implementation, the processing vessel may include at least one displacement fluid and at least one processing solution. The collector may also expel at least one displacement fluid from the processing vessel, such that the at least one displacement fluid pushes the target material into the collector.
1 . A collector comprising:
a primary body including a first end and an opposing second end;
a funnel located within the primary body and having a mouth at the second end that narrows to an apex located in the interior of the primary body;
a chamber having an opening at the first end that transitions to a seat located within the interior of the primary body; and
a cannula that extends from the apex of the funnel into the chamber, the cannula to allow fluids to pass between the funnel and the chamber.
2 . The collector of claim 1 , further comprising a ridge that protrudes radially and extends circumferentially around the primary body.
3 . The collector of claim 1 , the primary body further comprising a window to access at least a portion of the chamber.
4 . The collector of claim 1 , wherein the cannula is a tube.
5 . The collector of claim 1 , wherein the cannula includes a tapered end that at least partially extends in the chamber.
6 . A collector comprising:
a primary body including a first end and an opposing second end, the primary body including:
a conical-shaped opening in the second end that narrows to an apex within the primary body, and
a cavity with an opening at the first end; and
a cannula that extends from the apex into the cavity, the cannula to provide an opening between the conical-shaped opening and the cavity.
7 . The collector of claim 6 , further comprising a ridge that protrudes radially and extends circumferentially around the primary body.
8 . The collector of claim 6 , the primary body further comprising a window to access at least a portion of the cavity.
9 . The collector of claim 6 , wherein the cannula is a tube.
10 . The collector of claim 6 , wherein the cannula includes a tapered end that at least partially extends in the cavity.
11 . A system for retrieving a target material from a suspension stored in a primary vessel, the system comprising:
a processing vessel;
a collector having a first opening and a second opening connected by a cannula that extends partially into the second opening, the collector dimensioned to fit within an opening of the primary vessel; and
a displacement fluid to be placed in the processing vessel, wherein when the collector is placed in contact with the suspension through the opening of the primary vessel and the processing vessel is inserted in the first opening such that the cannula extends into the processing vessel, centrifugation of the primary vessel, collector, and processing vessel causes the displacement fluid to exchange places with a fraction of the suspension via the cannula.
12 . The system of claim 11 , the collector comprising:
a primary body including a first end and an opposing second end, the primary body including:
a conical-shaped opening in the second end that narrows to an apex within the primary body, and
a cavity with an opening at the first end; and
a cannula that extends from the apex into the cavity, the cannula to provide an opening between the conical-shaped opening and the cavity.
13 . The system of claim 12 , the processing vessel further comprising:
the at least one processing vessel comprising:
a first open end,
a second closed end, and
a plug in the second closed end, the plug to be re-sealable such that the plug can be repeatedly punctured with the cannula,
14 . The system of claim 12 , the collector further comprising a ridge extending circumferentially around the primary body.
15 . The system of claim 12 , the collector further comprising at least one window in the primary body to access at least a portion of the cavity.
16 . The system of claim 11 , the at least one displacement fluid to have a density greater than the target material.
17 . The system of claim 11 , the at least one displacement fluid to have a density less than the target material.
18 . The system of claim 13 , the at least one processing vessel further comprising a flexible cap to close the first open end.
19 . The system of claim 11 , further comprising:
a primary vessel having an open end to receive the suspension and the collector;
a float to be inserted within the primary vessel; and,
a sealing ring to tighten around the primary vessel to collapse the primary vessel inwardly until a seal is formed between the primary vessel and the float, the sealing ring to create a force directed toward a central axis of the primary vessel to cause the primary vessel to collapse inwardly.
20 . The system of claim 14 , further comprising:
a primary vessel having an open end to receive the suspension and the collector; and
a lock ring to apply to the outside of the primary vessel at the location of the ridge to inhibit movement of the collector relative to the primary vessel when the collector is inserted into the primary vessel.