Sequence amplification with linear primers
The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.
1. A method of amplifying a nucleic acid sequence in a parallel sequencing technique, said method comprising:
a) hybridizing a plurality of target nucleic acid sequences with a plurality of first linear primers which contain (i) a terminal 3′ region having a first random sequence and (ii) a 5′ region having a first universal sequence,
wherein the first random sequences of the terminal 3′ region include inosine, adenosine, guanosine, and cytidine, and the first random sequences exclude thymidine,
wherein the plurality of the first linear primers include different random sequences,
wherein the random sequences hybridize to a sequence of the target nucleic acid sequences, and
wherein the plurality of the first linear primers include the same universal sequence which does not hybridize to the target sequences; and
b) forming a plurality of extended linear primer products by extending the first linear primers that are hybridized to the target nucleic acid sequence;
c) hybridizing the plurality of the extended linear primer products to a plurality of second linear primers which contain (i) a terminal 3′ region having a second random sequence and (ii) a 5′ region having a second universal sequence,
wherein the second random sequences of the terminal 3′ region include inosine, adenosine, guanosine, and cytidine, and the second random sequences exclude thymidine,
wherein the plurality of the second linear primers include different random sequences, and
wherein the random sequences hybridize to a portion of the extended linear primer products,
wherein the plurality of the second linear primers include the same universal sequence as the universal sequence in the first linear primers; and
d) forming a plurality of double-extended linear primer products by extending the plurality of second linear primers that are hybridized to the plurality of the extended linear primer products.
2. The method of claim 1 , wherein the random sequence in the 3′ region is 4-12 nucleotides in length.
3. The method of claim 1 , wherein the target nucleic acid sequences are genomic DNA sequences.
4. The method of claim 1 , further comprising: amplifying the plurality of double-extended linear primer products in a single primer PCR reaction with a plurality of amplification primers that hybridize to the first or second universal sequence regions of the plurality of double-extended linear primer products.
5. The method of claim 1 , further comprising: forming a plurality of hairpin structures by self-hybridizing the plurality of double-extended linear primer products in step (d).
6. The method of claim 1 , further comprising: sequencing the double-extended linear primer products in step (d).
7. The method of claim 4 , further comprising: forming a plurality of hairpin structures by self-hybridizing the plurality of double-extended linear primer products.
8. The method of claim 7 , further comprising: sequencing the plurality of hairpin structures.