Reduced risk tobacco products and methods of making same
View Patent ↗Embodiments provided herein concern tobacco and tobacco products having a reduced amount of a harmful compound. More specifically, several embodiments concern approaches to modify the expression of a gene that is involved in the production of a harmful compound in tobacco, tobacco products made using these approaches and methods of determining whether the removal of said compounds using said approaches yields a tobacco and/or a tobacco product that has a reduced potential to contribute to a tobacco-related disease.
1. A cured transgenic tobacco comprising a first heterologous nucleic acid molecule, wherein said nucleic acid molecule comprises:
a) SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 33; or
b) a complement of the nucleic acid sequence of SEQ ID NO: 26, SEQ ID NO: 27 or SEQ ID NO: 33.
2. The cured transgenic tobacco of claim 1 , wherein said cured transgenic tobacco comprises an amount of nornicotine that is less than or equal to 0.2 mg/g of total biomass.
3. The cured transgenic tobacco of claim 1 , wherein said cured transgenic tobacco comprises an amount of nicotine that is less than or equal to 4 mg/g of total biomass.
4. The cured transgenic tobacco of claim 1 , wherein said cured transgenic tobacco comprises an amount of nicotine that is 2.17 to 3.99 mg/g of total biomass and an amount of nornicotine that is less than or equal to 0.00 to 0.06 mg/g of total biomass.
5. The cured transgenic tobacco of claim 1 , wherein said cured transgenic tobacco comprises a collective content of TSNAs of less than or equal to 5.0 μg/g, 4.0 μg/g, 3.0μg/g, 2.0 μg/g, 1.0 μg/g, 0.5 μg/g, or 0.2 μg/g of total biomass.
6. The cured transgenic tobacco of claim 1 , wherein said transgenic tobacco comprises an amount of nicotine that is less than or equal to 1.0 mg/g, 0.6 mg/g, 0.3 mg/g, or 0.05 mg/g of total biomass and a collective content of TSNAs of less than or equal to 5.0 μg/g, 4.0 μg/g, 3.0 μg/g, 2.0 μg/g, 1.0 μg/g, 0.5 μg/g, or 0.2 μg/g of total biomass.
7. A seed, reproductive tissues, vegetative tissues, biomass, leaves, plant parts or progeny derived from the transgenic tobacco of claim 1 , wherein said seed, reproductive tissues, vegetative tissues, biomass, leaves, plant parts or progeny comprises said nucleic acid molecule.
8. A tobacco product derived from the transgenic tobacco of claim 1 , wherein said tobacco product comprises said nucleic acid molecule.
9. The tobacco product of claim 8 , further defined as a cigarette.