Spectroscopic analysis of nutrient materials for use in a cell culture process
View Patent ↗In some embodiments, aspects of the disclosure relate to methods of evaluating cell culture materials, for example, nutrient materials, or other materials that can be used in cell culture media.
1. A method of evaluating a nutrient material, the method comprising:
performing a Raman spectroscopic analysis on a sample of the nutrient material to obtain a Raman spectrum; and
identifying a value of a quality parameter based on the Raman spectrum, the quality parameter relating to the nutrient material or to a cell culture grown using the nutrient material, wherein the quality parameter is a Day 8 pH or a Peak 5 or a Lactate Production Integral.
2. The method of claim 1 , further comprising:
differentiating the nutrient material from a second nutrient material based on a difference between the Raman spectrum of the nutrient material and a second Raman spectrum of the second nutrient material.
3. The method of claim 1 , further comprising:
performing a comparison of at least a portion of the Raman spectrum with at least a portion of a model Raman spectrum.
4. The method of claim 3 , further comprising:
based on a result of the comparison, accepting the nutrient material for use in a cell culture process.
5. The method of claim 3 , further comprising:
based on a result of the comparison, rejecting the nutrient material for use in a cell culture process.
6. The method of claim 1 , further comprising:
performing a comparison of data obtained from the Raman spectrum with model data.
7. The method of claim 1 , wherein:
performing the Raman spectroscopic analysis comprises performing Surface Enhanced Raman Spectroscopy (SERS).
8. The method of claim 1 , wherein:
performing the Raman spectroscopic analysis comprises performing ultraviolet (UV) Raman spectroscopy.
9. The method of claim 1 , further comprising:
performing a second spectroscopic analysis on a sample of the nutrient material to obtain a second spectrum; and
performing a data fusion analysis to evaluate the Raman spectrum.
10. The method of claim 9 , wherein the second spectroscopic analysis is a Nuclear Magnetic Resonance (NMR) analysis or a Near Infrared Spectroscopy (NIR) analysis.
11. The method of claim 1 , wherein the method further comprises predicting a value of the quality parameter based on a predictive least squares (PLS) predictive model.
12. A method of evaluating a nutrient material, the method comprising:
performing a Raman spectroscopic analysis on a sample of the nutrient material to obtain a Raman spectrum; and identifying a value of a quality parameter based on the Raman spectrum, the quality parameter relating to the nutrient material or to a cell culture grown using the nutrient material, wherein the quality parameter is a Day 8 pH.
13. A method of evaluating a nutrient material, the method comprising: performing a Raman spectroscopic analysis on a sample of the nutrient material to obtain a Raman spectrum; and identifying a value of a quality parameter based on the Raman spectrum, the quality parameter relating to the nutrient material or to a cell culture grown using the nutrient material, wherein the quality parameter is a Peak 5.
14. A method of evaluating a nutrient material, the method comprising: performing a Raman spectroscopic analysis on a sample of the nutrient material to obtain a Raman spectrum; and identifying a value of a quality parameter based on the Raman spectrum, the quality parameter relating to the nutrient material or to a cell culture grown using the nutrient material, wherein the quality parameter is a Lactate Production Integral.