Polypeptides having alpha-glucosidase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
1. A nucleic acid construct comprising a gene encoding a protein operably linked to a nucleotide sequence encoding a signal peptide selected from the group consisting of: nucleotides 1 to 42 of SEQ ID NO: 1, nucleotides 1 to 145 of SEQ ID NO: 3, and nucleotides 1 to 57 of SEQ ID NO: 5, wherein the gene is heterologous to the nucleotide sequence encoding the signal peptide.
2. A recombinant expression vector comprising the nucleic acid construct of claim 1 .
3. A recombinant host cell comprising the nucleic acid construct of claim 1 .
4. A method for producing a protein comprising (a) cultivating the recombinant host cell of claim 3 under conditions conducive for production of the protein; and (b) recovering the protein.