Methods for degrading nucleic acid
View Patent ↗Methods for degrading contaminant nucleic acid. The methods use combinations of metal ions and peroxide ions to produce a variety of oxidative species that degrade nucleic acid. Methods of the invention are useful for decontaminating laboratory equipment or solutions. After the equipment or solutions have been decontaminated, the metal ion and peroxide ion solution can be deactivated by raising the temperature to dissociate the peroxide or by binding the metal ions, e.g., with a chelating agent.
1. A method for degrading nucleic acid, the method comprising:
contacting nucleic acid with a solution comprising adding metal ions and peroxide ions to degrade the nucleic acid, such that the nucleic acid is not available for amplification; and
binding the metal ions subsequent to degradation of the nucleic acid.
2. The method of claim 1 ,
wherein the metal ion to peroxide ion molar ratio is from about 1:1 to about 1:25.
3. The method of claim 1 , wherein the metal ion to peroxide ion molar ratio is from about 1:5 to about 1:10.
4. The method of claim 1 , wherein the nucleic acid is associated with a separation medium or a solid support.
5. The method of claim 4 , wherein the solid support is a bead.
6. The method of claim 5 , wherein the bead is a type selected from the group consisting of magnetic, paramagnetic, ferromagnetic, and a combination thereof.
7. The method of claim 4 , wherein the solid support is a planar substrate.
8. The method of claim 4 , further comprising removing the separation media or support from the solution and subsequently rinsing the separation media or support with an aqueous solution.
9. The method of claim 1 , wherein the metal ions are selected from the group consisting of iron, manganese, copper, nickel, and cobalt.
10. The method of claim 9 , wherein the metal ions are Fe 2+ ions or Fe 3+ ions or a combination thereof.
11. The method of claim 1 , wherein the solution comprises hydrogen peroxide.
12. The method of claim 1 , wherein the solution has a pH of about 3 to about 12.
13. The method of claim 12 , wherein the solution has a pH of about 3 to about 6.
14. The method of claim 1 , wherein the solution has a concentration of about 1 mM or greater of metal ions.
15. The method of claim 1 , wherein contacting lasts for at least about 10 minutes.
16. The method of claim 15 , wherein contacting lasts for at least about 60 minutes.
17. The method of claim 1 , further comprising heating the solution to dissociate peroxide ions.
18. The method of claim 17 , wherein the temperature of the solution is raised to at least about 40° C.
19. The method of claim 1 , wherein binding comprises contacting the solution with an ion-exchange medium or chelating agent.
20. The method of claim 1 , wherein the solution is an aqueous solution comprising hydrogen peroxide and Fe 2 (SO 4 ) 3 .
21. The method of claim 1 , wherein binding comprises contacting the solution with an ion-exchange medium or chelating agent.
22. A method for degrading nucleic acid, such that the nucleic acid is unavailable for amplification, the method comprising:
contacting nucleic acid with a solution comprising metal ions and peroxide ions; and
heating the solution to dissociate the peroxide ions for at least about 15 minutes; and
binding the metal ions subsequent to degradation of the nucleic acid.
23. The method of claim 22 , wherein a temperature of the solution is raised to at least about 40° C.
24. The method of claim 22 , wherein binding comprises contacting the solution with an ion-exchange medium or chelating agent.
25. The method of claim 22 , wherein the metal ions are Fe 2+ ions or Fe 3+ ions or a combination thereof.
26. The method of claim 22 , further comprising adjusting a pH of the solution to between about 3 and 12.