Process for the production of ethanol and solvents from lignocellulosic biomass with recycling of an ethanolic liquor obtained from the fermentation of pentoses
View Patent ↗Embodiments provided here relate to second generation processes for the production of alcohols or solvents, wherein the lignocellulosic or cellulosic biomass undergoes a pre-treatment before being converted into ethanol after an enzymatic hydrolysis and fermentation. The glucidic polymers of the pre-treated plant material are hydrolysed by cellulases. The alcoholigenic microorganisms used for the ethanolic fermentation principally use hexoses, for example, glucose and mannose. The alcohols of the liquor, with or without separation of the suspended material are extracted by distillation. The stillage, containing pentoses which have not been used, are fermented into an ex-pentose ethanolic liquor and at least a portion of the liquor is recycled upstream of the enzymatic hydrolysis. The enzymatic hydrolysis and the ethanolic fermentation are thus carried out separately or simultaneously in the presence of a portion of the ethanolic liquor, which mixture does not interfere with the performance of the enzymes and microorganisms.
1. A process for the production of alcohols or solvents or both alcohols and solvents from a cellulosic or lignocellulosic biomass, comprising:
a) thermochemically pretreating a cellulosic or lignocellulosic substrate to produce a pre-treated substrate;
b) optionally washing said pre-treated substrate and adjusting the pH of said pre-treated substrate to produce a washed substrate;
c) enzymatically hydrolyzing said pre-treated substrate and/or optionally washed substrate, with cellulolytic or hemicellulolytic enzymes or both cellulolytic and hemicellulolytic enzymes to produce a hydrolysate and a water-insoluble residue;
d) fermenting hexoses contained in said hydrolysate of step c) into ethanol and/or solvents by an alcoholigenic microorganism to obtain an ex-hexose ethanolic liquor;
e) extracting said ethanol or solvent in said ex-hexose ethanolic liquor by:
e1) separating and purifying said ethanol or solvents or both ethanol and solvents obtained from step d); and
e2) separating a solid cake containing a water-insoluble residue and obtaining stillage comprising non-fermented pentoses; and
f) fermenting said pentoses contained in said stillage of step e2 with a pentose-fermenting microorganism and obtaining an ex-pentose ethanolic liquor, wherein at least a portion of said ex-pentose ethanolic liquor obtained in step f) is recycled to step c) or step d), and wherein said microorganisms used in steps d) and f) are different microorganisms.
2. The process of claim 1 , wherein said pretreating in step a) is performed under acidic conditions.
3. The process of claim 1 , wherein said pentose fermenting microorganism of step f) is Pichia stipitis or Candida shehatae.
4. The process of claim 1 , wherein steps a) and b) are carried out simultaneously at a temperature in the range of 30° C. to 45° C. and at a pH in the range of 4 to 6.
5. The process of claim 1 , wherein step e2) is coupled with washing said solid cake.
6. The process of claim 1 , wherein said stillage sent to said fermenting of step f) has a sugar concentration in the range of 30 to 90 g/L of sugars.
7. The process of claim 1 , wherein all of said stillage obtained in step e2) is sent to step f) and a portion of said ex-pentose ethanolic liquor which is not recycled is sent to a water/solvent separation step before being sent to said extracting of step e).
8. The process of claim 1 , wherein only a portion of said stillage is sent to step f).
9. The process of claim 1 , wherein at least a portion of said ex-pentose ethanolic liquor obtained in step f) is recycled to step d).
10. The process of claim 1 , wherein said cellulolytic and/or hemicellulolytic enzymes of step c) are produced by a microorganism of the genus Trichoderma, Aspergillus, Penicillium , or Schizophyllum , or an anaerobic bacterium of the genus Clostridium.
11. The process of claim 1 , said alcoholigenic microorganism of step d) is a yeast.
12. The process of claim 11 , wherein said yeast is Schizosaccharomyces pombe, Saccharomyces uvarum or diastaticus , or Kluyveromyces fragilis .