Green process for producing polyhydroxyalkanoates and chemicals using a renewable feedstock
View Patent ↗Disclosed are organisms genetically engineered to make useful products when grown on ethanol as a carbon source. The organisms are genetically engineered to produce various useful products such as polyhydroxyalkanoates, diols, diacids, higher alcohols, and other useful chemicals.
1. An organism that is genetically engineered to express an alcohol dehydrogenase and an aldehyde dehydrogenase and to convert ethanol to acetyl-CoA when grown on ethanol as a sole carbon source,
wherein the organism is genetically engineered to express A. baylyi ADP1 genes ACIAD2015 and ACIAD2018,
wherein the organism is further genetically engineered to produce a polyhydroxyalkanoate polymer.
2. The organism of claim 1 , wherein the organism produces polyhydroxyalkanoate polymer when grown on ethanol as a sole carbon source.
3. The organism of claim 1 , wherein the polyhydroxyalkanoate polymer is selected from the group consisting of: polyglycolic acid (PGA), poly-3-hydroxybutyrate (P3HB), poly-3-hydroxypropionate (P3HP), poly-4-hydroxybutryrate (P4HB), poly-5-hydroxyvalerate (P5HV), poly-3-hydroxybutyrate-co-4-hydroxybutyrate (P(3HB-co-4HB)), poly-3-hydroxybutyrate-co-5-hydroxyvalerate (P(3HB-co-5HV)), and copolymers thereof.
4. The organism of claim 1 , wherein the organism is selected from the group consisting of: Escherichia coli, Ralstonia eutropha, Acinetobacter baumannii, Acinetobacter baylyi, Acinetobacter aceti, Acinetobacter sp. DRI, Acinetobacter calcoaceticus, Acinetobacter haemolyticus, Acinetobacter johnsonii, Acinetobacter junii, Acinetobacter lwoffii, Acinetobacter radioresistens, Acinetobacter venetianus, Acinetobacter sp. DSM, Zoogloea ramigera, Allochromatium vinosum, Rhodococcus ruber, Delftia acidovorans, Aeromonas caviae, Synechocystis sp. PCC 6803 , Synechococcus elongatus PCC 7942 , Thiocapsa pfenigii, Bacillus megaterium, Clostridium kluyveri, Methylobacterium extorquens, Nocardia corralina, Nocardia salmonicolor, Pseudomonas fluorescens, Pseudomonas oleovorans, Pseudomonas sp. 6-19, Pseudomonas sp. 61-3 and Pseudomonas putida, Rhodobacter sphaeroides, Alcaligenes latus, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kiuyveromyces iactis, Kiuyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Chiarella spp., Chiarella minutissima, Chiarella emersonii, Chiarella sorokiniana, Chiarella ellipsoidea, Chiarella sp., and Chiarella protothecoides.
5. The organism of claim 1 , wherein the organism if further genetically engineered to delete a native genomic copy of gene gabD (Succinate semialdehyde dehydrogenase, NADP+-dependent) and a native genomic copy of gene yneI (Succinate semialdehyde dehydrogenase, NAD(P)+-dependent).
6. The organism of claim 5 , wherein the organism is further genetically engineered to express a C. kluyveri gene org (CoA-transferase).