Methods of Diagnosing Neuropathic Pain
The invention includes compositions and methods useful for the diagnosis, assessment, and characterization of neuropathic pain in a subject in need thereof, based upon the expression level of at least one miRNA or snoRNA that is associated with neuropathic pain.
1 . A method of diagnosing neuropathic pain in a subject, the method comprising:
a. obtaining a biological sample from the subject,
b. determining the level of at least one microRNA or snoRNA in the biological sample,
c. comparing the level of the at least one microRNA or snoRNA in the biological sample with the level of the at least one miRNA or snoRNA in a comparator, wherein when the level of the at least one microRNA or snoRNA in the biological sample is different than the level of the at least one miRNA or snoRNA in the comparator, the subject is diagnosed with neuropathic pain.
2 . The method of claim 1 , wherein the neuropathic pain is complex regional pain syndrome (CRPS).
3 . The method of claim 1 , wherein the at least one micro RNA or snoRNA is at least one selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
4 . The method of claim 1 , wherein the at least one microRNA or snoRNA is at least two microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
5 . The method of claim 1 , wherein the at least one micro RNA or snoRNA is at least three microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
6 . The method of claim 1 , wherein the subject is human.
7 . The method of claim 1 , further comprising the step of treating the subject for neuropathic pain.
8 . The method of claim 1 , wherein the comparator is at least one comparator selected from the group consisting of positive control, a negative control, a normal control, a wild-type control, a historical control, and a historical norm.
9 . The method of claim 1 , wherein the level of the at least one miRNA or snoRNA is higher than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, by at least 100%, by at least 125%, by at least 150%, by at least 175%, by at least 200%, by at least 250%, by at least 300%, by at least 400%, by at least 500% by at least 600%, by at least 700%, by at least 800%, by at least 900%, by at least 1000%, by at least 1500%, by at least 2000%, or by at least 5000%.
10 . The method of claim 1 , wherein the level of the at least one miRNA or snoRNA is lower than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, or by at least 100%.
11 . The method of claim 1 , wherein determining the level of the at least one microRNA or snoRNA utilizes at least one technique selected from the group consisting of reverse transcription, PCR and a microarray.
12 . A method of determining the severity of neuropathic pain in a subject, the method comprising:
a. obtaining a biological sample from the subject,
b. determining the level of at least one microRNA or snoRNA in the biological sample,
c. comparing the level of the at least one microRNA or snoRNA in the biological sample with the level of the at least one miRNA or snoRNA in a comparator, wherein the greater the difference between the level of the at least one microRNA or snoRNA in the biological sample and the level of the at least one miRNA or snoRNA in the comparator, the greater the severity of neuropathic pain.
13 . The method of claim 12 , wherein the neuropathic pain is complex regional pain syndrome (CPRS).
14 . The method of claim 12 , wherein the at least one microRNA or snoRNA is at least one microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
15 . The method of claim 12 , wherein the at least one microRNA or snoRNA is at least two microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
16 . The method of claim 12 , wherein the at least one microRNA or snoRNA is at least three microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
17 . The method of claim 12 , wherein the subject is human.
18 . The method of claim 12 , further comprising the step of treating the subject for neuropathic pain.
19 . The method of claim 12 , further comprising the step of stratifying the subject for inclusion in a clinical trial based upon the severity of the subject's neuropathic pain.
20 . The method of claim 12 , wherein the comparator is at least one comparator selected from the group consisting of positive control, a negative control, a normal control, a wild-type control, a historical control, and a historical norm.
21 . The method of claim 12 , wherein the level of the at least one miRNA or snoRNA is higher than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, by at least 100%, by at least 125%, by at least 150%, by at least 175%, by at least 200%, by at least 250%, by at least 300%, by at least 400%, by at least 500% by at least 600%, by at least 700%, by at least 800%, by at least 900%, by at least 1000%, by at least 1500%, by at least 2000%, or by at least 5000%.
22 . The method of claim 12 , wherein the level of the at least one miRNA or snoRNA is lower than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, or by at least 100%.
23 . The method of claim 12 , wherein determining the level of the at least one microRNA or snoRNA utilizes at least one technique selected from the group consisting of reverse transcription, PCR and a microarray.
24 . A method of evaluating the progression of neuropathic pain in a subject, the method comprising:
a. obtaining a biological sample from the subject,
b. determining the level of at least one microRNA or snoRNA in the biological sample at a first time point,
c. comparing the level of the at least one microRNA or snoRNA in the biological sample at the first time point with the level of the at least one miRNA or snoRNA in a comparator,
d. determining the level of at least one microRNA or snoRNA in the biological sample at a second time point,
e. comparing the level of the at least one microRNA or snoRNA in the biological sample at the second time point with the level of the at least one miRNA or snoRNA in a comparator,
f. wherein when the difference in the level of the at least one microRNA or snoRNA is the biological sample at the second time point, as compared with the comparator, is greater than the difference in the level of the at least one microRNA or snoRNA in the biological sample at the first time point, as compared with the comparator, the neuropathic pain is progressing.
25 . The method of claim 24 , wherein the neuropathic pain is complex regional pain syndrome (CPRS).
26 . The method of claim 24 , wherein the at least one microRNA or snoRNA is at least one microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
27 . The method of claim 24 , wherein the at least one microRNA or snoRNA is at least two microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
28 . The method of claim 24 , wherein the at least one microRNA or snoRNA is at least three microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
29 . The method of claim 24 , wherein the subject is human.
30 . The method of claim 24 , further comprising the step of treating the subject for neuropathic pain.
31 . The method of claim 24 , further comprising the step of modifying the subject's treatment for neuropathic pain.
32 . The method of claim 24 , wherein the comparator is at least one comparator selected from the group consisting of a positive control, a negative control, a normal control, a wild-type control, a historical control, and a historical norm.
33 . The method of claim 24 , wherein the level of the at least one miRNA or snoRNA is higher than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, by at least 100%, by at least 125%, by at least 150%, by at least 175%, by at least 200%, by at least 250%, by at least 300%, by at least 400%, by at least 500% by at least 600%, by at least 700%, by at least 800%, by at least 900%, by at least 1000%, by at least 1500%, by at least 2000%, or by at least 5000%.
34 . The method of claim 24 , wherein the level of the at least one miRNA or snoRNA is lower than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, or by at least 100%.
35 . The method of claim 24 , wherein determining the level of the at least one microRNA or snoRNA utilizes at least one technique selected from the group consisting of reverse transcription, PCR and a microarray.
36 . A method of evaluating a treatment of neuropathic pain in a subject in need thereof, the method comprising:
a. obtaining a biological sample from the subject,
b. determining the level of at least one microRNA or snoRNA in the biological sample at a first time point,
c. comparing the level of the at least one microRNA or snoRNA in the biological sample at the first time point with the level of the at least one miRNA or snoRNA in a comparator,
d. determining the level of at least one microRNA or snoRNA in the biological sample at a second time point,
e. comparing the level of the at least one microRNA or snoRNA in the biological sample at the second time point with the level of the at least one miRNA or snoRNA in a comparator,
f. wherein when the difference in the level of the at least one microRNA or snoRNA is the biological sample at the second time point, as compared with the comparator, is greater than the difference in the level of the at least one microRNA or snoRNA in the biological sample at the first time point, as compared with the comparator, the treatment of neuropathic pain is reducing neuropathic pain.
37 . The method of claim 36 , wherein the neuropathic pain is complex regional pain syndrome (CPRS).
38 . The method of claim 36 , wherein the at least one micro RNA, or snoRNA is at least one microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
39 . The method of claim 36 , wherein the at least one microRNA or snoRNA is at least two microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
40 . The method of claim 36 , wherein the at least one microRNA or snoRNA is at least three microRNA selected from the group consisting of hsa-miR-939, hsa-miR-25#, hsa-let-7c, hsa-let-7a, hsa-let-7b, hsa-miR-320B, hsa-miR-126, hsa-miR-629.A, hsa-miR-664, hsa-miR-320, hsa-miR-1285, hsa-miR-625#, hsa-miR-532-3p, hsa-miR-181a-2#, RNU48, hsa-miR-720, RNU44, and hsa-miR-1201.
41 . The method of claim 36 , wherein the subject is human.
42 . The method of claim 36 , further comprising the step of continuing to treat the subject for neuropathic pain.
43 . The method of claim 36 , further comprising the step of modifying the subject's treatment for neuropathic pain.
44 . The method of claim 36 , wherein the comparator is at least one comparator selected from the group consisting of a positive control, a negative control, a normal control, a wild-type control, a historical control, and a historical norm.
45 . The method of claim 36 , wherein the level of the at least one miRNA or snoRNA is higher than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, by at least 100%, by at least 125%, by at least 150%, by at least 175%, by at least 200%, by at least 250%, by at least 300%, by at least 400%, by at least 500% by at least 600%, by at least 700%, by at least 800%, by at least 900%, by at least 1000%, by at least 1500%, by at least 2000%, or by at least 5000%.
46 . The method of claim 36 , wherein the level of the at least one miRNA or snoRNA is lower than the level of the at least one miRNA or snoRNA in the comparator by at least 10%, by at least 20%, by at least 30%, by at least 40%, by at least 50%, by at least 60%, by at least 70%, by at least 80%, by at least 90%, or by least 100%.
47 . The method of claim 36 , wherein determining the level of the at least one microRNA or snoRNA utilizes at least one technique selected from the group consisting of reverse transcription, PCT and a microarray.