Granted Patent B2

US 9,273,366 · App. 14/119,563 · Granted Mar 1, 2016

Abstract

Claims (12)

1. A composition comprising:

(a) an oligonucleotide comprising the sequence of SEQ ID NO:10; and

(b) a first forward primer comprising the sequence of SEQ ID NO:14 and a first reverse primer comprising the sequence of SEQ ID NO:15, wherein the first forward primer and the first reverse primer each is capable of annealing to a target sequence under a PCR condition to thereby amplify the target sequence.

2. The composition of claim 1 , further comprising an oligonucleotide probe comprising the sequence of SEQ ID NO:6, a second forward primer comprising the sequence of SEQ ID NO:16 and a second reverse primer comprising the sequence of SEQ ID NO:17.

3. A kit comprising the composition of claim 2 .

4. A method for amplifying a target sequence, comprising:

(a) contacting the composition of claim 1 with the target sequence; and

(b) performing a PCR using the target sequence as template, the forward primer and the reverse primer, whereby the target sequence is amplified.

5. A kit comprising the composition of claim 1 .

6. A method for determining human immunodeficiency virus (HIV) in a sample, comprising:

(a) performing a PCR with a nucleic acid template in the sample using a forward primer comprising the sequence of SEQ ID NO:14 and a reverse primer comprising the sequence of SEQ ID NO:15, whereby an amplicon is generated; and

(b) detecting the amplicon with an oligonucleotide comprising the sequence of SEQ ID NO:10, wherein the detection of the amplicon is indicative of the presence of HIV in the sample.

Assignments (25)