Agglutination enhancer
View Patent ↗The purpose of the present invention is to provide an agglutination enhancer which shows superior agglutination enhancing effect to those of conventional immunoagglutination enhancers, and the present invention relates to an agglutination enhancer for an immunoagglutination measurement method which comprises a polymer having a monomer unit shown by the following general formula [1]: (Wherein R 1 represents a hydrogen atom or a methyl group; R 2 and R 3 independently represent a methyl group or an ethyl group, respectively; X represents —NH— or an oxygen atom; n represents an integer of 1 to 6; and m represents an integer of 1 to 3), and an immunoagglutination measurement method in which, in the coexistence of the above-described agglutination enhancer for immunoagglutination measurement method, an antibody against analyte or an antigen for the analyte is brought into contact with the analyte to cause an antigen-antibody reaction.
1. A method of measuring agglutination, which comprises
bringing an antibody or an antigen against an analyte into contact with the analyte, to cause an antigen-antibody reaction, in the presence of a polymer that is either
(a) a homopolymer consisting of a monomer unit of formula [1]:
wherein R 1 is a hydrogen atom or a methyl group; R 2 and R 3 are a methyl group, X is —NH— or an oxygen atom; n is an integer of 2 to 4; and m is an integer of 1
or
(b) a copolymer consisting of
(i) a monomer unit of formula [1]:
wherein R 1 is a hydrogen atom or a methyl group; R 2 and R 3 are a methyl group, X is —NH— or an oxygen atom; n is an integer of 2 to 4; and m is an integer of 1, and
(ii) a monomer unit of formula [2]:
wherein R 4 is a hydrogen atom or a methyl group, and k is an integer of 4 to 8, to generate agglutination and form particles from the antigen-antibody reaction, and
measuring the degree of agglutination of the particles based on a change of scattered light or transmitted light.
2. The method according to claim 1 , wherein the analyte is C-reactive protein (CRP), serum ferritin (Fer), prostate specific antigen (PSA), or creatine kinase-MB (CK-MB).
3. The method according to claim 1 , wherein the content of the monomer unit of formula [1] in the copolymer is 50% by mole or more and less than 100% by mole.
4. The method according to claim 1 , wherein the polymer has a weight-average molecular weight of 50,000 to 3,000,000 g/mol.
5. A method of measuring agglutination, which comprises
bringing an antibody or an antigen against an analyte into contact with the analyte, to cause an antigen-antibody reaction, in the presence of a polymer that is either
(a) a homopolymer consisting of a monomer unit that is
or
(b) a copolymer consisting of
(i) a monomer unit that is
and
(ii) a monomer unit of formula [2-1-1]:
to generate agglutination and form particles from the antigen-antibody reaction, and
measuring the degree of agglutination of the particles based on a change of scattered light or transmitted light.