Protein comprising truncated form of extracellular region protein of Frizzled 2, and pharmaceutical composition for treating bone diseases which comprises said protein
View Patent ↗The present invention provides a protein comprising a truncated form of an extracellular region protein of Frizzled 2, which has higher secretion activity than that of a known protein comprising an extralellular cysteine-rich domain of Frizzled 2 in a production cell and bone mass-increasing activity higher than or equal to that of the known protein, or DNA encoding said protein.
1. A fusion protein of
(a) a protein comprising amino acids from the amino acid Q at position 1 of the N-terminal to any one amino acid of A at position 153, G at position 154, G at position 155, T at position 156, P at position 157, G at position 158, G at position 159, P at position 160, G at position 161, G at position 162, G at position 163, G at position 164, A at position 165, P at position 166, P at position 167, R at position 168, Y at position 169, A at position 170, T at position 171, L at position 172, Eat position 173, H at position 174, or P at position 175 of the amino acid sequence of SEQ ID NO:1; and
(b) a mammal-derived immunoglobulin Fc protein or a variant of the mammal-derived immunoglobulin Fc protein.
2. The fusion protein according to claim 1 , wherein the mammal-derived immunoglobulin Fc protein consists of the amino acid sequence of SEQ ID NO:3.
3. A fusion protein comprising the amino acid sequence of any one of SEQ ID NOs: 72 to 94.
4. A pharmaceutical composition comprising the fusion protein according to claim 1 .
5. A DNA encoding the fusion protein according to claim 1 .
6. The DNA according to claim 5 , wherein nucleotide sequence encoding the mammal-derived immunoglobulin Fc protein is the nucleotide sequences of SEQ ID NO:4.
7. A DNA consisting of the nucleotide sequence of any one of SEQ ID NOs: 42 to 64.
8. A pharmaceutical composition comprising the DNA according to claim 5 .
9. A pharmaceutical composition comprising the fusion protein of claim 3 .
10. A DNA encoding the fusion protein according to claim 1 .
11. A pharmaceutical composition comprising the DNA according to claim 10 .