Systems and methods for acid recycle
Methods and systems for pretreating lignocellulosic biomass are disclosed. An acid solution between 1% to 1.6% sulfuric acid is applied to the biomass. The biomass is subjected to an elevated temperature to cause the production of xylose, glucose, and furfural. Adjustments to temperature, acid concentration, and time can generate at least 80% or 90% of theoretical xylose, 45% or 50% of the theoretical glucose, and less than 4000 ppm of furfural in the xylose liquor. A portion of the resulting xylose liquor may be separated from the glucan solids. The xylose liquor, still highly acidic, can be recycled to reduce subsequent acid loading requirements. Makeup acid solution is added to the xylose liquor and subsequent biomass to ensure a proper solids to liquids ratio. The biomass is again treated to higher temperatures to yield sugars. The process may be repeated for each subsequent cycle.
1. A method for pretreating lignocellulosic biomass, comprising:
a) applying an acid solution to a first portion of biomass feedstock, wherein the biomass feedstock comprises ground corn cobs and corn stover, and wherein the acid solution comprises between about 1% to 1.6% sulfuric acid;
b) maintaining an elevated temperature of the first portion of biomass feedstock to hydrolyze hemicellulose present in the biomass feedstock such that a xylose yield of greater than about 80% of theoretical is achieved, wherein a liquid xylose liquor comprising furfural in an amount of 4000 ppm or less and a glucan solid are produced from the first portion of biomass feedstock while maintaining the elevated temperature, wherein the maintaining the elevated temperature comprises maintaining the elevated temperature within the range of about 120° C. to 150° C.;
c) before step “h”, separating at least a portion of the liquid xylose liquor from the glucan solid;
d) recycling a fraction of liquid xylose liquor that is separated from the glucan solid and applying the fraction of the liquid xylose liquor to a subsequent portion of the biomass feedstock, wherein the fraction of the liquid xylose liquor that is recycled is 70% or more of the total xylose liquor that is separated from the glucan solid;
e) applying a makeup acid solution to the subsequent portion of the biomass feedstock;
f) maintaining an elevated temperature of the subsequent portion of the biomass feedstock such that xylose yield of greater than about 80% of theoretical is achieved, wherein a liquid xylose liquor and a glucan solid are produced from the subsequent portion of the biomass feedstock while maintaining the elevated temperature, wherein the maintaining the elevated temperature comprises maintaining the elevated temperature within the range of about 120° C. to 150° C.;
g) repeating steps c) through f) at least three times; and
h) enzymatically hydrolyzing the glucan solid to produce glucose.
2. The method of claim 1 , further comprising releasing sugar glucose.
3. The method of claim 2 , wherein at step b) and step f) greater than about 45% of theoretical glucose yield is achieved.
4. The method of claim 2 , wherein at step b) and step f) greater than about 50% of theoretical glucose yield is achieved.
5. The method of claim 1 , wherein the maintaining the elevated temperature comprises maintaining the elevated temperature for at least about 10 minutes.
6. The method of claim 1 , wherein the maintaining the elevated temperature comprises maintaining the elevated temperature for less than about 120 minutes.
7. The method of claim 1 , wherein at step b) and step f) greater than about 90% of theoretical xylose yield is achieved.
8. The method of claim 1 , wherein the liquid xylose liquor comprises less than about 3000 ppm furfural.
9. The method of claim 1 , wherein step g) is repeated at least five times.
10. The method of claim 1 , wherein step g) is repeated for about ten cycles.
11. A method for pretreating lignocellulosic biomass to be supplied to a fermentation system for production of a fermentation product, comprising:
a) applying a xylose liquor to biomass feedstock, wherein the biomass feedstock comprises ground corn cobs and corn stover;
b) applying a makeup acid solution to the biomass feedstock;
c) maintaining an elevated temperature of the biomass feedstock to hydrolyze hemicellulose present in the biomass feedstock such that a xylose yield of greater than about 80% of theoretical is achieved, wherein xylose liquor comprising furfural in an amount of 4000 ppm or less and a glucan solid are produced from the biomass feedstock while maintaining the elevated temperature, wherein the maintaining the elevated temperature comprises maintaining the elevated temperature within the range of about 120° C. to 150° C.;
d) before step “f”, separating at least some portion of the xylose liquor from the glucan solid, and recycling a fraction of liquid xylose liquor that is separated from the glucan solid and applying the fraction of the liquid xylose liquor to a subsequent portion of the biomass feedstock, wherein the fraction of the liquid xylose liquor that is recycled is 70% or more of the total xylose liquor that is separated from the glucan solid;
e) repeating steps a) through d) at least three times; and
f) enzymatically hydrolyzing the glucan solid.