GENETICALLY MODIFIED PHAGE AND USE THEREOF
The present invention relates to a genetically modified phage and use thereof in a method for producing a bio molecule of interest.
1 .- 15 . (canceled)
16 . A genetically modified phage comprising:
an inserted expression system;
an inactivated S, R and/or Q gene; and
an inactivated Int and/or Xis gene.
17 . The phage of claim 16 , comprising an inactivated Rz gene.
18 . The phage of claim 16 , comprising an inactivated Int gene, an inactivated Xis gene, and inactivated R, S and Rz genes.
19 . The phage of claim 18 , further comprising an inactivated Q gene.
20 . The phage of claim 16 , wherein the inserted expression system is a T7 expression system.
21 . The phage of claim 16 , wherein the phage is a genetically modified lambda phage, 434 phage, phi80 phage, phi81 phage, HK97 phage or P21 phage.
22 . The phage of claim 16 , wherein the phage is genetically modified phage of any of phages P11 to P53.
23 . A kit comprising:
a phage of claim 16 ; and
a helper phage.
24 . A host cell comprising a phage of claim 16 .
25 . The host cell of claim 24 , wherein the host cell is an enterobacteria.
26 . The host cell of claim 24 , wherein the host cell is E. coli.
27 . The host cell of claim 26 , wherein the host cell is E. coli BL21.
28 . The host cell of claim 24 , further comprising at least one inactivated tonA, galK, araB, araA, lon, ompT, rcsA, hsdR, mrr, endA, and/or recA gene.
29 . The host cell of claim 24 , further comprising an inserted ccdb gene.
30 . A kit comprising:
a host cell of claim 29 ; and
a plasmid comprising the ccdA gene.
31 . A process for preparing a host cell, comprising infecting a host cell with a phage of claim 16 .
32 . A process for producing a biomolecule of interest, comprising:
cultivating a host cell comprising a phage of claim 16 and a nucleic acid sequence of the biomolecule of interest; and
recovering the biomolecule of interest.