Solubilized phospholipids for stabilizing nucleic acid polymerases
View Patent ↗Compositions and methods are provided that relate to solubilized phospholipids and their use in stabilizing nucleic acid polymerases. For example, a phospholipid with a tail containing at least 8 carbons can be solubilized in the presence of an amphipathic molecule.
1. A method comprising:
providing a thermostable polymerase in a buffer and a solubilized long-chain phospholipid, wherein the solubilized phospholipid has at least 8 to 30 carbons; and
stabilizing the thermostable polymerase.
2. A method according to claim 1 , wherein the solubilized long-chain phospholipid is solubilized by adding an amphipathic molecule.
3. A method according to claim 2 , wherein the solubulizer is a lipid having a side chain of no more than 8 carbons.
4. A method according to claim 1 , further comprising:
enhancing the activity of the polymerase in the presence of the solubilized phospholipid as can be measured in a replication assay by incorporation of deoxynucleoside triphosphate.