IP Library Granted Patent US 9,221,794
Granted Patent B2
US 9,221,794 · App. 14/136,691 · Granted Dec 29, 2015

PRMT5 inhibitors and uses thereof

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Quick Facts
Patent No.
US 9,221,794
App. No.
14/136,691
Granted
Dec 29, 2015
Kind
B2
Abstract

Described herein are compounds of Formula (I), pharmaceutically acceptable salts thereof, and pharmaceutical compositions thereof. Compounds of the present invention are useful for inhibiting PRMT5 activity. Methods of using the compounds for treating PRMT5-mediated disorders are also described.

Claims (41)

1. A compound of Formula (I A ):

or a pharmaceutically acceptable salt thereof,

wherein:

R 1 is hydrogen, R z , or —C(O)R z , wherein R z is optionally substituted C 1-6 alkyl;

X A is a bond, —O—, —N(R)—, —CR 4A R 5A —, —O—CR 4A R 5A , —N(R)—CR 4A R 5A —, —O—CR 4A R 5A —O—, —N(R)—CR 4A R 5A —O—, —N(R)—CR 4A R 5A N(R)—, —O—CR 4A R 5A N(R)—, —CR 4A R 5A —O—, —CR 4A R 5A N(R)—, —O—CR 4A R 5A —CR 6A R 7A —, —N(R)—CR 4A R 5A —CR 6A R 7A —, —CR 6A R 7A —CR 4A R 5A —O—, —CR 6A R 7A —CR 4A R 5A —N(R)—, or —CR 6A R 7A —CR 4A R 5A —;

each R is independently hydrogen or optionally substituted C 1-6 aliphatic;

R 2A and R 3A are independently selected from the group consisting of hydrogen, halo, —CN, —NO 2 , optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted phenyl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —OR A , —N(R B ) 2 , —SR A , —C(═O)R A , —C(O)OR A , —C(O)SR A , —C(O)N(R B ) 2 , —C(O)N(R B )N(R B ) 2 , —OC(O)R A , —OC(O)N(R B ) 2 , —NR B C(O)R A , —NR B C(O)N(R B ) 2 , —NR B C(O)N(R B )N(R B ) 2 , —NR B C(O)OR A , —SC(O)R A , —C(═NR B )R A , —C(═NNR B )R A , —C(═NOR A )R A , —C(═NR B )N(R B ) 2 , —NR B C(═NR B )R B , —C(═S)R A , —C(═S)N(R B ) 2 , —NR B C(═S)R A , —S(O)R A , —OS(O) 2 R A , —SO 2 R A , —NR B SO 2 R A , and —SO 2 N(R B ) 2 ; or R 2A and R 3A are taken together with their intervening atoms to form an optionally substituted carbocyclic or heterocyclic ring;

R 4A and R 5A are independently selected from the group consisting of hydrogen, halo, —CN, —NO 2 , optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted phenyl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —OR A , —N(R B ) 2 , —SR A , —C(═O)R A , —C(O)OR A , —C(O)SR A , —C(O)N(R B ) 2 , —C(O)N(R B )N(R B ) 2 , —OC(O)R A , —OC(O)N(R B ) 2 , —NR B C(O)R A , —NR B C(O)N(R B ) 2 , —NR B C(O)N(R B )N(R B ) 2 , —NR B C(O)OR A , —SC(O)R A , —C(═NR B )R A , —C(═NNR B )R A , —C(═NOR A )R A , —C(═NR B )N(R B ) 2 , —NR B C(═NR B )R B , —C(═S)R A , —C(═S)N(R B ) 2 , —NR B C(═S)R A , —S(O)R A , —OS(O) 2 R A , —SO 2 R A , —NR B SO 2 R A , and —SO 2 N(R B ) 2 ; or R 4A and R 5A are taken together with their intervening atoms to form an optionally substituted carbocyclic or heterocyclic ring;

R 6A and R 7A are independently selected from the group consisting of hydrogen, halo, —CN, —NO 2 , optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted phenyl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —OR A , —N(R B ) 2 , —SR A , —C(═O)R A , —C(O)OR A , —C(O)SR A , —C(O)N(R B ) 2 , —C(O)N(R B )N(R B ) 2 , —OC(O)R A , —OC(O)N(R B ) 2 , —NR B C(O)R A , —NR B C(O)N(R B ) 2 , —NR B C(O)N(R B )N(R B ) 2 , —NR B C(O)OR A , —SC(O)R A , —C(═NR B )R A , —C(═NNR B )R A , —C(═NOR A )R A , —C(═NR B )N(R B ) 2 , —NR B C(═NR B )R B , —C(═S)R A , —C(═S)N(R B ) 2 , —NR B C(═S)R A , —S(O)R A , —OS(O) 2 R A , —SO 2 R A , —NR B SO 2 R A , and —SO 2 N(R B ) 2 ; or R 6A and R 7A are taken together with their intervening atoms to form an optionally substituted carbocyclic or heterocyclic ring;

each R A is independently selected from the group consisting of hydrogen, optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl;

each R B is independently selected from the group consisting of hydrogen, optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or two R B groups are taken together with their intervening atoms to form an optionally substituted heterocyclic ring;

R 8A , R 9A , R 10A , and R 11A are independently hydrogen, halo, or optionally substituted aliphatic;

Cy A is a monocyclic or bicyclic, saturated, partially unsaturated, or aromatic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein Cy A is substituted with 0, 1, 2, 3, or 4 R y groups;

each R y is independently selected from the group consisting of halo, —CN, —NO 2 , optionally substituted aliphatic, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, —OR A , —N(R B ) 2 , —SR A , —C(═O)R A , —C(O)OR A , —C(O)SR A , —C(O)N(R B ) 2 , —C(O)N(R B )N(R B ) 2 , —OC(O)R A , —OC(O)N(R B ) 2 , —NR B C(O)R A , —NR B C(O)N(R B ) 2 , —NR B C(O)N(R B )N(R B ) 2 , —NR B C(O)OR A , —SC(O)R A , —C(═NR B )R A , —C(═NNR B )R A , —C(═NOR A )R A , —C(═NR B )N(R B ) 2 , —NR B C(═NR B )R B , —C(═S)R A , —C(═S)N(R B ) 2 , —NR B C(═S)R A , —S(O)R A , —OS(O) 2 R A , —SO 2 R A , —NR B SO 2 R A , and —SO 2 N(R B ) 2 ;

each R x is independently selected from the group consisting of halo, —CN, optionally substituted aliphatic, and —OR′;

R′ is hydrogen or optionally substituted aliphatic; and

n is 0, 1, 2, 3, 4, 5, 6, 7, or 8.

2. The compound of claim 1 , wherein the compound is:

or a pharmaceutically acceptable salt thereof.

3. A pharmaceutical composition comprising a compound of claim 1 or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

4. A kit or packaged pharmaceutical comprising a compound of claim 1 or a pharmaceutically acceptable salt thereof, and instructions for use thereof.

5. The compound of claim 1 , wherein the compound is of Formula (I A -a):

or a pharmaceutically acceptable salt thereof.

6. The compound of claim 1 , wherein the compound is of Formula (I A -b):

or a pharmaceutically acceptable salt thereof.

7. The compound of claim 1 , wherein the compound is of Formula (II A ):

or a pharmaceutically acceptable salt thereof.

8. The compound of claim 1 , wherein the compound is of Formula (III A ):

or a pharmaceutically acceptable salt thereof.

9. The compound of claim 1 , wherein R 1 is hydrogen.

10. The compound of claim 1 , wherein n is 0.

11. The compound of claim 1 , wherein R 2A and R 3A are each hydrogen.

12. The compound of claim 1 , wherein Cy A is phenyl, optionally fused to a phenyl, carbocyclyl, heterocyclyl, or heteroaryl ring, wherein the point of attachment is on the phenyl ring, and wherein Cy A is substituted with 0, 1, 2, 3, or 4 R y groups.

13. The compound of claim 1 , wherein Cy A is substituted with at least one R y group selected from the group consisting of halo, —CN, —OR A , —N(R B ) 2 , C 1-6 alkyl, —C(O)N(R B ) 2 , —SO 2 R A , —N(R B )C(O)R A , —N(R B )SO 2 R A , —SO 2 N(R B ) 2 , and —C(O)R A .

14. The compound of claim 13 , wherein Cy A is substituted with one R y group.

15. The compound of claim 1 , wherein the compound is of Formula (IV A ):

or a pharmaceutically acceptable salt thereof.

16. The compound of claim 1 , wherein the compound is of Formula (V A ):

or a pharmaceutically acceptable salt thereof.

17. The compound of claim 1 , wherein the compound is:

or a pharmaceutically acceptable salt thereof.

Assignments (3)
TERMINATION AND RELEASE OF SECURITY INTEREST IN PATENTS AT REEL/FRAME: 051057/0848 Recorded Aug 13, 2022
From: BIOPHARMA CREDIT PLC
To: EPIZYME, INC.
Reel/Frame 061165/0501 →
SECURITY INTEREST Recorded Nov 19, 2019
From: EPIZYME, INC.
To: BIOPHARMA CREDIT PLC
Reel/Frame 051057/0848 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 17, 2014
From: DUNCAN, KENNETH W.; CHESWORTH, RICHARD; BORIACK-SJODIN, PAULA ANN; MUNCHHOF, MICHAEL JOHN
To: EPIZYME, INC.
Reel/Frame 031994/0784 →