IP Library › Granted Patent US 9,200,310
Granted Patent B2
US 9,200,310 · App. 14/137,608 · Granted Dec 1, 2015

High fidelity restriction endonucleases

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Quick Facts
Patent No.
US 9,200,310
App. No.
14/137,608
Granted
Dec 1, 2015
Kind
B2
Abstract

Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.

Claims (2)

1. A composition comprising a variant MluI restriction endonuclease having reduced star activity, wherein the variant MluI restriction endonuclease comprises an amino acid sequence that differs from the amino acid sequence of the parent MluI restriction endonuclease by two amino acid substitutions at positions corresponding to positions 112 and 132 of SEQ ID NO:28.

2. A composition according to claim 1 , wherein the amino acid substitutions are E112A and R132A.

Assignments (2)
NOTICE OF GRANT OF SECURITY INTEREST IN PATENTS Recorded Sep 27, 2023
From: NEW ENGLAND BIOLABS, INC.
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 065044/0729 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 2, 2015
From: ZHU, ZHENYU; QUIMBY, AINE; GUAN, SHENGXI; SUN, DAPENG; HUANG, YISHU; LAI, XUHUI; CHAN, SIU-HONG; LI, XIANGHUI; XU, SHUANG-YONG; ZHANG, CHUNHUA
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 035067/0976 →