IP Library › Granted Patent US 9,150,901
Granted Patent B2
US 9,150,901 · App. 14/137,660 · Granted Oct 6, 2015

High fidelity restriction endonucleases

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Quick Facts
Patent No.
US 9,150,901
App. No.
14/137,660
Granted
Oct 6, 2015
Kind
B2
Abstract

Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.

Claims (2)

1. A composition comprising a variant HindIII restriction endonuclease having reduced star activity, wherein the variant HindIII restriction endonuclease comprises an amino acid sequence that differs from the amino acid sequence of the parent HindIII restriction endonuclease by an amino acid substitution at a position corresponding to position 148 in SEQ ID NO:2.

2. The composition of claim 1 , wherein the substitution is Q148I.

Assignments (2)
NOTICE OF GRANT OF SECURITY INTEREST IN PATENTS Recorded Sep 27, 2023
From: NEW ENGLAND BIOLABS, INC.
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 065044/0729 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 2, 2015
From: ZHU, ZHENYU; QUIMBY, AINE; GUAN, SHENGXI; SUN, DAPENG; HUANG, YISHU; LAI, XUHUI; CHAN, SIU-HONG; LI, XIANGHUI; XU, SHUANG-YONG; ZHANG, CHUNHUA
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 035067/0549 →