Microarray for delivery of therapeutic agent and methods of use
Devices and methods for using and manufacturing microstructure arrays having at least a detachable distal area are described.
1. A microstructure apparatus comprising:
a backing having a first surface and a second surface opposed thereto;
a microstructure array comprising a plurality of microstructures extending outwardly from the first surface of the backing;
the plurality of microstructures comprising a biodegradable distal layer and at least one proximal layer positioned between the distal layer and the first surface of the backing;
the distal layer comprised of at least one therapeutic agent and a polymer matrix comprising at least one polymer, wherein:
(i) the at least one polymer is a hydrophobic polymer that is insoluble or substantially insoluble in aqueous solvents, wherein the polymer matrix is comprised of about 50-100% of the hydrophobic polymer, thereby imparting a brittle character to the distal layer and promoting detachment of at least a portion of the distal layer; or
(ii) the at least one polymer is at a concentration in a polymer casting solution used to form the distal layer of the plurality of microstructures that is at or below an entanglement concentration (C E ) for the at least one polymer, thereby promoting detachment of at least a portion of the distal layer; and
wherein the distal layer formed from the polymer matrix having the configuration of (i) or (ii) is configured such that at least the portion of the distal layer detaches from the plurality of microstructures upon insertion of the microstructure array into skin.
2. The microstructure apparatus of claim 1 , wherein the at least one therapeutic agent is a small molecule drug at least partially soluble with the at least one polymer in a solvent or co-solvent.
3. The microstructure apparatus of claim 1 , where the at least one therapeutic agent is dispersed in the distal layer.
4. The microstructure apparatus of claim 1 , where at least about 10% of the distal layer detaches readily upon the insertion of the array into the skin.
5. The microstructure apparatus of claim 1 , where at least about 50% of the distal layer detaches readily upon the insertion of the array into the skin.
6. The microstructure apparatus of claim 1 , where at least about 70% of the distal layer detaches readily upon the insertion of the array into the skin.
7. The microstructure apparatus of claim 1 , where at least about 90% of the distal layer detaches readily upon the insertion of the array into the skin.
8. The microstructure apparatus of claim 1 , where about 100% of the distal layer detaches readily upon the insertion of the array into the skin.
9. The microstructure apparatus of claim 1 , where at least a portion of the plurality of microstructures have a first cross-dimensional diameter that is larger than a second cross-dimensional diameter.
10. The microstructure apparatus of claim 9 , where at least a portion of the plurality of microstructures have a cross-dimensional shape selected from the group consisting of a diamond, a rectangle, and an oval.
11. The microstructure apparatus of claim 9 , where at least a portion of the plurality of microstructures have a cross-dimensional shape selected from rectangular, square, oval, elliptical, circular, and polygonal.
12. The microstructure apparatus of claim 1 , wherein at least a portion of the at least one proximal layer, at least a portion of the distal layer, or at least portions of both the at least one proximal layer and the distal layer have a funnel shape.
13. The microstructure apparatus of claim 1 , wherein the plurality of microstructures are affixed to the first surface of the backing.
14. The microstructure apparatus of claim 1 , wherein at least a portion of the backing forms the at least one proximal layer.
15. The microstructure apparatus of claim 1 , further comprising a substrate having a first surface and a second surface opposed thereto, where the second surface of the backing is affixed to the first surface of the substrate.
16. The microstructure apparatus of claim 1 , where the at least one polymer of the distal layer comprises at least one hydrophobic polymer and about 0-50% of a hydrophilic polymer.
17. The microstructure apparatus of claim 1 , where the at least one polymer of the distal layer comprises at least one hydrophobic polymer and about 0-20% of a hydrophilic polymer.
18. The microstructure apparatus of claim 1 , wherein about 1-99% of the distal layer is comprised of the at least one therapeutic agent.
19. The microstructure apparatus of claim 18 , wherein 1-90% of the distal layer is comprised of the at least one therapeutic agent.
20. The microstructure apparatus of claim 18 , wherein the at least one therapeutic agent is a small molecule drug at least partially soluble with the at least one polymer in a solvent or co-solvent.
21. The microstructure apparatus of claim 20 , wherein about 1-50% of the distal layer is comprised of the small molecule drug.
22. The microstructure apparatus of claim 20 , wherein about 1-40% of the distal layer is comprised of the small molecule drug.
23. The microstructure apparatus of claim 20 , wherein about 10-30% of the distal layer is comprised of the small molecule drug.
24. The microstructure apparatus of claim 18 , where the at least one therapeutic agent is dispersed in the distal layer.
25. The microstructure apparatus of claim 24 , wherein about 1-90% of the distal layer is comprised of the at least one dispersed therapeutic agent.
26. The microstructure apparatus of claim 24 , wherein about 1-50% of the distal layer is comprised of the at least one dispersed therapeutic agent.
27. The microstructure apparatus of claim 24 , wherein about 10-40% of the distal layer is comprised of the at least one dispersed therapeutic agent.
28. The microstructure apparatus of claim 24 , wherein about 20-30% of the distal layer is comprised of the at least one dispersed therapeutic agent.
29. The microstructure apparatus of claim 1 , wherein the at least one polymer has a glass transition temperature (Tg) above about body temperature.
30. The microstructure apparatus of claim 1 , wherein the at least one polymer has a molecular weight of about 1-100K Da.
31. The microstructure apparatus of claim 1 , wherein at least about 10-100% of the distal layer is configured to detach readily upon the insertion of the array into the skin.
32. The microstructure apparatus of claim 1 , wherein the hydrophobic polymer is insoluble in aqueous solvents.
33. A method of making the microstructure apparatus of claim 1 , comprising:
dissolving or suspending a therapeutic agent in a solvent to form a therapeutic agent solution or suspension;
dissolving at least one polymer in a solvent to form a polymer solution;
mixing the therapeutic agent solution or suspension and the polymer solution or suspension to form a polymer matrix solution or suspension;
dispensing the polymer matrix solution or suspension on a mold having an array of microstructure cavities; followed by pressurization;
removing excess solution or suspension polymer matrix on the mold surface; and drying the matrix; and
dispensing a basement or backing layer on the mold surface; and
drying the basement or backing layer.
34. The method of claim 33 , further comprising:
affixing the basement or backing layer to a substrate.
35. The method of claim 34 further comprising:
using a nonwoven or porous film double coated with adhesive to affix the basement or backing layer to a substrate.