Oil composition and method of recovering same
View Patent ↗The present invention generally relates to oil compositions and methods of producing such oil compositions. More particularly, the present invention relates to an oil composition recovered from a fermentation product as well as methods of recovering such oil compositions for use in various processes such as bio-diesel production as well as in various edible applications.
1. A method of recovering an oil composition from a fermentation process comprising the steps of
fermenting a starch composition to form a fermented product comprising an oil fraction and a first aqueous portion;
separating an oil fraction from the first aqueous portion, wherein the oil fraction comprises the oil composition and from 10% to 60% moisture w/w moisture based on the total weight of the separated oil fraction;
adjusting the pH of the oil fraction to produce the oil composition and a second aqueous portion; and
recovering the oil composition from the pH adjusted oil fraction, such that the recovered oil composition comprises no greater than about 2% moisture.
2. The method of claim 1 , wherein the recovered oil composition comprises a free fatty acid content of no greater than 5% w/w based on the total weight of the oil composition.
3. The method of claim 1 , wherein the recovered oil composition comprises an iodine value of not greater than 125.
4. The method of claim 1 , wherein the recovered oil composition comprises a combined moisture and insoluble content of no greater than 1% w/w based on the total weight of the oil composition.
5. The method of claim 1 , wherein the recovered oil composition has an unsaponifiables content of no greater than 3% based on the total weight of the composition.
6. The method of claim 1 , wherein the recovered oil composition comprises at least one component selected from the group consisting of:
a lutein content of at least 50 mcg/g,
a zeaxanthin content of at least 30 mcg/g,
a cis-lutein/zeaxanthin content of at least 10 mcg/g,
an alpha-cryptoxanthin content of at least 5 mcg/g,
a beta-cryptoxanthin content of at least 5 mcg/ g,
an alpha-carotene content of at least 0.5 mcg/g,
a beta-carotene content of at least 1 mcg/g,
a cis-beta-carotene content of at least 0 .1 mcg/g,
an alpha-tocopherol content of at least 50 mcg/g,
a beta-tocopherol content of at least 2 mcg/g,
a gamma-tocopherol content of at least 300 mcg/g,
a delta-tocopherol content of at least 15 mcg/g,
an alpha-tocotrienol content of at least 50 mcg/g,
a beta-tocotrienol content of at least 5 mcg/g,
a gamma-tocotrienol content of at least 80 mcg/g,
a delta-tocotrienol content of at least 5 mcg/g.
7. The method of claim 1 , comprising the steps of
saccharifying the starch composition, without cooking, with an enzyme composition to form a saccharified composition; and
fermenting the saccharified composition to yield the fermented product.
8. The method of claim 7 , wherein the starch composition is hydrolyzed at a pH of 4.0 to 5.0.
9. The method of claim 7 , wherein the starch composition that is saccharified is in a slurry comprising water and 5% to 60% dried solids.
10. The method of claim 7 , wherein the starch composition is held at a temperature of 0° C. to 20° C. below initial gelatinization temperature of the starch composition for a period of 5 minutes to 12 hours during saccharifying and before fermenting.
11. The method of claim 1 , wherein the fermented product is beer, whole stillage, thin stillage or syrup.
12. The method of claim 1 , wherein the step of separating an oil fraction from the fermented product is accomplished by applying a first centrifugal force to the oil fraction.
13. The method of claim 12 , wherein applying the first centrifugal force forms an emulsion.
14. The method of claim 1 , wherein the separated oil fraction comprises from 10% to 40% w/w protein based on the total weight of the separated oil fraction.
15. The method of claim 1 , wherein the separated oil fraction comprises from 20% to 70% w/w oil based on the total weight of the separated oil fraction.
16. The method of claim 1 , wherein the pH is adjusted to a range of from 7 to 10.
17. The method of claim 1 , further comprising the step of applying a second centrifugal force to the oil fraction after adjusting the pH.
18. The method of claim 17 , further comprising the step of removing the second aqueous portion after applying the second centrifugal force.