Electrospun fibers for protein stabilization and storage
View Patent ↗An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.
1. A biochemical storage medium, consisting essentially of one or more fibers, wherein the one or more fibers have a nanoscale to microscale diameter and comprise one or more biologically active components, the one or more biologically active components comprise one or more proteins, the fibers comprise one or more of carbohydrates, stabilizing factors, or nucleotides, and the carbohydrates comprise one or more of polysucrose, melezitose, sucrose, trehalose, or sorbitol, wherein the one or more fibers comprise ficoll and are soluble in aqueous solutions.
2. The biochemical storage medium of claim 1 , wherein the one or more fibers have diameters between about 10 nm and 2000 nm.
3. The biochemical storage medium of claim 1 , wherein the one or more proteins comprise an enzyme or an antibody that retain biological activity when the one or more fibers are dissolved.
4. The biochemical storage medium of claim 1 , wherein the stabilizing factors comprise one or more of albumin, polyethylene glycol, or polyvinyl alcohol.
5. The biochemical storage medium of claim 1 , wherein the one or more biologically active components comprise one or more of labile small molecules, dNTP's, rNTP's, detergents, salts, divalent cations, buffer molecules, primers, flavin adenine dinucleotide (FAD), nicotinamide adenine dinucleotide (NAD), dye conjugated esters, or labeled molecules.
6. The biochemical storage medium of claim 1 , wherein the one or more biologically active components in the fibers are stable at room temperature for at least a week.
7. The biochemical storage medium of claim 1 , wherein the fibers correspond to a quantity of the one or more biologically active components suitable for use in a preconfigured reaction.
8. The biochemical storage medium of claim 1 , wherein the ficoll has an average molecular weight of 400,000.
9. The biochemical storage medium of claim 1 , wherein the ficoll is 70% of the one or more fibers.
10. A biochemical storage medium, consisting essentially of fibers each having a nanoscale to microscale diameter and comprising one or more biologically active components, the one or more biologically active components comprise one or more proteins, the fibers comprising one or more of carbohydrates, stabilizing factors, or nucleotides, and the carbohydrates comprise one or more of polysucrose, melezitose, sucrose, trehalose, or sorbitol, wherein the fibers include ficoll and are soluble in aqueous solutions.
11. The biochemical storage medium of claim 10 , wherein the one or more fibers have diameters between about 10 nm and 2000 nm.
12. The biochemical storage medium of claim 10 , wherein the one or more proteins comprise an enzyme or an antibody that retain biological activity when the one or more fibers are dissolved.
13. The biochemical storage medium of claim 10 , wherein the one or more biologically active components comprise one or more of labile small molecules, dNTP's, rNTP's, detergents, salts, divalent cations, buffer molecules, primers, flavin adenine dinucleotide (FAD), nicotinamide adenine dinucleotide (NAD), dye conjugated esters, or labeled molecules.
14. The biochemical storage medium of claim 10 , wherein the one or more biologically active components in the fibers are stable at room temperature for at least a week.
15. The biochemical storage medium of claim 10 , wherein the fibers correspond to a quantity of the one or more biologically active components suitable for use in a preconfigured reaction.
16. The biochemical storage medium of claim 10 , wherein the fibers comprise ficoll having an average molecular weight of 400,000.
17. The biochemical storage medium of claim 16 , wherein the ficoll is 70% of the fibers.