IP Library Granted Patent US 9,663,449
Granted Patent B2
US 9,663,449 · App. 14/149,496 · Granted May 30, 2017

Lipid containing formulations

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Quick Facts
Patent No.
US 9,663,449
App. No.
14/149,496
Granted
May 30, 2017
Kind
B2
Abstract

Compositions and methods useful in administering nucleic acid based therapies, for example association complexes such as liposomes and lipoplexes are described.

Claims (45)

1. A compound of formula (X)

wherein

R 1 and R 2 are each independently H, C 1 -C 6 alkyl, optionally substituted with 1-4 R 5 , C 2 -C 6 alkenyl, optionally substituted with 1-4 R 5 , or C(NR 6 )(NR 6 ) 2 ;

R 3 and R 4 are each independently alkyl, alkenyl, alkynly, each of which is optionally substituted with fluoro, chloro, bromo, or iodo;

L 1 and L 2 are each independently —NR 6 C(O)—, —C(O)NR 6 —, —S—S—, —N(R 6 )C(O)N(R 6 )—, —OC(O)N(R 6 )—, —N(R 6 )C(O)O—, —O—N═C—, OR, —OC(O)NH—N═C—, or —NHC(O)NH—N═C—, or

L 1 -R 3 and L 2 -R 4 can be taken together to form an acetal, a ketal, or an orthoester, wherein R 3 and R 4 are defined as above and can also be H or phenyl;

R 5 is fluoro, chloro, bromo, iodo, —OR 7 , —N(R 8 )(R 9 ), —CN, SR 10 , S(O)R 10 , or S(O) 2 R 10 ;

R 6 is H or C 1 -C 6 alkyl;

R 7 is H or C 1 -C 6 alkyl;

R 8 and R 9 are independently H or C 1 -C 6 alkyl;

R 10 is H or C 1 -C 6 alkyl;

m is 1, 2, 3, 4, 5, or 6;

n is 0, 1, 2, 3, 4, 5, or 6;

or a pharmaceutically acceptable salt thereof.

2. The compound of claim 1 , wherein R 1 is H, methyl, ethyl, isopropyl, or 2-hydroxyethyl and R 2 is H, methyl, ethyl, propyl, or isopropyl.

3. The compound of claim 1 , wherein both m and n are 1.

4. The compound of claim 1 , wherein both L 1 and L 2 are —N(R 6 )C(O)N(R 6 )—.

5. The compound of claim 1 , wherein both L 1 and L 2 are —OC(O)N(R 6 )— or —N(R 6 )C(O)O—.

6. The compound of claim 1 , wherein L 1 is —NR 6 C(O)— and L 2 is —S—S—.

7. The compound of claim 1 , wherein L 1 is —OC(O)N(R 6 ) or —N(R 6 )C(O)O— and L 2 is —S—S—.

8. The compound of claim 1 , wherein L 1 is —N(R 6 )C(O)N(R 6 )— and L2 is —S—S—.

9. The compound of claim 1 , wherein L 1 -R 3 and L 2 -R 4 are taken together to form an acetal, a ketal, or an orthoester.

10. The compound of claim 1 , wherein both R 3 and R 4 are C 6 -C 28 alkyl.

11. The compound of claim 10 , wherein each L 1 and L 2 are independently —S—S—, —OC(O)N(R 6 )— or —N(R 6 )C(O)O—.

12. The compound of claim 1 , wherein each R 3 and R 4 are independently C 6 -C 30 alkenyl.

13. The compound of claim 1 , wherein at least one of R 3 and R 4 is provided in formula (II) below

wherein

x is an integer from 1 to 8; and

y is an integer from 1-10.

14. The compound of claim 1 , wherein at least one of R 1 and R 2 is provided in formula (III) below

wherein

x is an integer from 1 to 8; and

y is an integer from 1-10.

15. The compound of claim 1 , wherein at least one of R 1 and R 2 is provided in formula (IV) below

wherein

x is an integer from 1 to 8; and

y is an integer from 1-10.

16. The compound of claim 1 , wherein at least one of R 1 and R 2 is provided in formula (V) below

wherein

x is an integer from 1 to 8; and

y is an integer from 1-10.

17. A preparation comprising the compound of claim 1 , or a pharmaceutically acceptable salt thereof.

18. A compound of Formula 3

or a pharmaceutically acceptable salt thereof.

19. A preparation comprising the compound of claim 18 , or a pharmaceutically acceptable salt thereof.

Assignments (4)
MERGER AND CHANGE OF NAME Recorded Jul 27, 2018
From: PROTIVA BIOTHERAPEUTICS INC.; ARBUTUS BIOPHARMA CORPORATION
To: ARBUTUS BIOPHARMA CORPORATION
Reel/Frame 046640/0764 →
CHANGE OF NAME Recorded Feb 14, 2017
From: TEKMIRA PHARMACEUTICALS CORPORATION
To: ARBUTUS BIOPHARMA CORPORATION
Reel/Frame 041255/0856 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 29, 2014
From: ALNYLAM PHARMACEUTICALS, INC.
To: TEKMIRA PHARMACEUTICALS CORPORATION
Reel/Frame 032779/0879 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 29, 2014
From: MANOHARAN, MUTHIAH; RAJEEV, KALLANTHOTTATHIL G.; AKINC, AKIIN; NARAYANANNAIR, JAYAPRAKASH K.; JAYARAMAN, MUTHUSAMY; MAIER, MARTIN
To: ALNYLAM PHARMACEUTICALS, INC.
Reel/Frame 032779/0916 →