Method for acquiring a heat-stable antibody-displayed phage

1. A method for acquiring a heat-stable antibody-displayed phage, the method comprising steps of:

(a) supplying an antibody-displayed phage library aqueous solution containing plural types of antibody-displayed phages to a support comprising a polypeptide on the surface thereof, so as to bind the plural types of the antibody-displayed phages to the polypeptide specifically; wherein

the polypeptide consists of an amino acid sequence represented by SEQ ID NO: 01;

each antibody-displayed phage comprises proteins containing a phagemid vector in the inside thereof;

each antibody-displayed phage comprises an antibody fragment on the external surface thereof; and

the phagemid vector has a gene sequence coding for the antibody fragment;

(b) heating the support to the temperature of not less than 37 degrees Celsius and not more than 70 degrees Celsius, so as to release a portion of the antibody-displayed phages from the support and so as to leave the other antibody-displayed phages on the support selectively; and

(c) collecting the other antibody-displayed phages which has been left on the support selectively in the step (b) so as to obtain the heat-stable antibody-displayed phage.

2. The method according to claim 1 , further comprising the following step between the step (a) and the step (b),

a step of removing an antibody-displayed phages which have not been bound to the polypeptide specifically in the step (a).

3. The method according to claim 1 , further comprising the following step between the step (b) and the step (c), a step of washing the support.

4. The method according to claim 1 , further comprising the following step after the step (c), a step of amplifying the heat-stable antibody-displayed phages collected in the step (c).

5. A method for acquiring a heat-stable antibody fragment, the method comprising steps of:

(a) supplying an antibody-displayed phage library aqueous solution containing plural types of antibody-displayed phages to a support comprising a polypeptide on the surface thereof, so as to bind the plural types of the antibody-displayed phages to the polypeptide specifically; wherein

the polypeptide consists of an amino acid sequence represented by SEQ ID NO: 01;

each antibody-displayed phage comprises proteins containing a phagemid vector in the inside thereof;

each antibody-displayed phage comprises an antibody fragment on the external surface thereof; and

the phagemid vector has a gene sequence coding for the antibody fragment;

(b) heating the support to the temperature of not less than 37 degrees Celsius and not more than 70 degrees Celsius, so as to release a portion of the antibody-displayed phages from the support and so as to leave the other antibody-displayed phages on the support selectively;

(c) collecting the other antibody-displayed phages which has been left on the support selectively in the step (b) so as to obtain the heat-stable antibody-displayed phage;

(d) bringing a cell into contact with the heat-stable antibody-displayed phages collected in the step (c) to produce an infected cell; and

(e) incubating the infected cell produced in the step (d) to acquire the heat-stable antibody fragment.

6. A heat-stable peptide consisting of the amino acid sequence represented by SEQ ID NO: 01.

7. The heat-stable peptide according to claim 6 , wherein the peptide is stable even when the peptide is heated to 70 degrees Celsius.