Protein glycosylation modification in methylotrophic yeast
View Patent ↗The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an α-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.
1. A genetically engineered Pichia strain, which lacks a functional enzyme involved in production of high mannose structures, and comprises an exogenous nucleic acid encoding and expressing an exogenous enzyme for production of Man 5 GlcNAc 2 , wherein said enzyme involved in production of high mannose structures is α-1,6-mannosyltransferase encoded by the OCH1 gene, said exogenous enzyme for production of Man 5 GlcNAc 2 is α-1,2-mannosidase or an enzymatically active fragment thereof and is targeted to the endoplasmic reticulum (ER), wherein said OCH1 gene is disrupted in said strain and the OCH1 gene disruption is the sole genetic disruption of genes coding for Golgi mannosyl transferases acting in N-glycosylation of said strain, and wherein said strain produces Man 5 GlcNAc 2 as a N-glycan structure or an intermediate N-glycan structure.
2. The strain of claim 1 , wherein said exogenous enzyme is of a fungal origin or a mammalian origin.
3. The strain of claim 1 , wherein the targeting of said exogenous enzyme to the ER is achieved by engineering said exogenous enzyme to include the ER retention signal as set forth in SEQ ID NO: 1.
4. A method for producing a glycoprotein with reduced hyperglycosylation in Pichia , comprising: (i) providing a Pichia strain, which lacks a functional enzyme involved in production of high mannose structures, and comprises an exogenous nucleic acid encoding and expressing an exogenous enzyme for production of Man 5 GlcNAc 2 , wherein said enzyme involved in production of high mannose structures is α-1,6-mannosyltransferase encoded by the OCH1 gene, said exogenous enzyme for production of Man 5 GlcNAc 2 is α-1,2-mannosidase or an enzymatically active fragment thereof and is targeted to the ER, wherein said OCH1 gene is disrupted in said strain and the OCH1 gene disruption is the sole genetic disruption of genes coding for Golgi mannosyl transferases acting in N-glycosylation of said strain, and wherein said strain produces Man 5 GlcNAc 2 as a N-glycan structure or an intermediate N-glycan structure; and (ii) producing said glycoprotein in said Pichia strain.
5. The method of claim 4 , wherein said exogenous enzyme is of a fungal origin or a mammalian origin.
6. The method of claim 4 , wherein the targeting of said exogenous enzyme to the ER is achieved by engineering said exogenous enzyme to include the ER retention signal as set forth in SEQ ID NO: 1.
7. The strain of claim 1 , wherein said α-1,2-mannosidase is T. reesei α-1,2-mannosidase, and said strain produces Man 5 GlcNAc 2 as a predominant N-glycan structure or a predominant intermediate N-glycan structure.
8. The method of claim 4 , wherein said α-1,2-mannosidase is T. reesei α-1,2-mannosidase, and said strain produces Man 5 GlcNAc 2 as a predominant N-glycan structure or a predominant intermediate N-glycan structure.