Interfering RNA delivery system and uses thereof
View Patent ↗The invention provides a delivery system comprising a cell penetrating peptide, 10 histidines, and an interfering RNA molecule. The system can be used for delivering interfering RNA molecules into a cell in vivo or in vitro. Therapeutic uses for the delivery system are also provided.
1. An interfering RNA delivery system comprising a molecular group consisting of an interfering RNA molecule linked to a YM3-10H peptide, wherein the YM3-10H peptide comprises an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 2)
CHHHHHTHRLPRRRRRRHHHHHC and
(SEQ ID NO: 3)
HHHHHTHRLPRRRRRRHHHHHC.
2. The delivery system of claim 1 , wherein the interfering RNA molecule is linked to the YM3-10H peptide via a covalent bond.
3. The delivery system of claim 1 , wherein the interfering RNA molecule is a short interfering RNA (siRNA), micro RNA (miRNA), or short hairpin RNA (shRNA).
4. A pharmaceutical composition comprising the delivery system of claim 1 and a pharmaceutically acceptable carrier.
5. A method of delivering an interfering RNA molecule into a cell, comprising:
(a) attaching an interfering RNA molecule to a YM3-10H peptide, wherein the YM3-10H peptide comprises an amino acid sequence selected from the group consisting of:
CHHHHHTHRLPRRRRRRHHHHHC
(SEQ ID NO: 2)
and
HHHHHTHRLPRRRRRRHHHHHC,
(SEQ ID NO: 3)
thereby forming an interfering RNA delivery system; and
(b) administering the interfering RNA delivery system to the cell.
6. The method of claim 5 , wherein the interfering RNA molecule is linked to the YM3-10H peptide via a covalent bond.
7. The method of claim 5 , wherein the interfering RNA molecule is a siRNA, miRNA, or shRNA.