Methods For Degrading or Converting Plant Cell Wall Polysaccharides
The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
1 . A method for degrading or converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products.
2 . The method of claim 1 , wherein the one or more heterologous genes encode enzymes selected from the group consisting of a cellulase, endoglucanase, cellobiohydrolase, and beta-glucosidase.
3 . The method of claim 1 , wherein the heterologous gene encodes a cellulase.
4 . The method of claim 1 , wherein the heterologous gene encodes an endoglucanase.
5 . The method of claim 1 , wherein the heterologous gene encodes a cellobiohydrolase.
6 . The method of claim 1 , wherein the heterologous gene encodes a beta-glucosidase.
7 . The method of claim 1 , wherein the one or more heterologous genes encode enzymes further selected from the group consisting of a glucohydrolase, xyloglucanase, xylanase, xylosidase, alpha-arabinofuranosidase, alpha-glucuronidase, acetyl xylan esterase, mannanase, mannosidase, alpha-galactosidase, mannan acetyl esterase, galactanase, arabinanase, pectate lyase, pectin lyase, pectate lyase, polygalacturonase, pectin acetyl esterase, pectin methyl esterase, alpha-arabinofuranosidase, beta-galactosidase, galactanase, arabinanase, alpha-arabinofuranosidase, rhamnogalacturonase, rhamnogalacturonan lyase, rhamnogalacturonan acetyl esterase, xylogalacturonosidase, xylogalacturonase, rhamnogalacturonan lyase, lignin peroxidase, manganese-dependent peroxidase, hybrid peroxidase, and laccase.
8 . The method of claim 1 , wherein the spent whole fermentation broth of the recombinant microorganism is supplemented by the addition of one or more enzymes selected from the group consisting of a cellulase, endoglucanase, cellobiohydrolase, and beta-glucosidase.
9 . The method of claim 1 , further comprising recovering the one or more products obtained from the degraded or converted plant cell wall polysaccharides.
10 . The method of claim 9 , wherein the product is a sugar.
11 . A method for producing one or more substances, comprising:
(a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material;
(b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and
(c) recovering the one or more organic substances from the fermentation.
12 . The method of claim 11 , wherein the one or more heterologous genes encode enzymes selected from the group consisting of a cellulase, endoglucanase, cellobiohydrolase, and beta-glucosidase.
13 . The method of claim 11 , wherein the heterologous gene encodes a cellulase.
14 . The method of claim 11 , wherein the heterologous gene encodes an endoglucanase.
15 . The method of claim 11 , wherein the heterologous gene encodes a cellobiohydrolase.
16 . The method of claim 11 , wherein the heterologous gene encodes a beta-glucosidase.
17 . The method of claim 11 , wherein the one or more heterologous genes encode enzymes further selected from the group consisting of a glucohydrolase, xyloglucanase, xylanase, xylosidase, alpha-arabinofuranosidase, alpha-glucuronidase, acetyl xylan esterase, mannanase, mannosidase, alpha-galactosidase, mannan acetyl esterase, galactanase, arabinanase, pectate lyase, pectin lyase, pectate lyase, polygalacturonase, pectin acetyl esterase, pectin methyl esterase, alpha-arabinofuranosidase, beta-galactosidase, galactanase, arabinanase, alpha-arabinofuranosidase, rhamnogalacturonase, rhamnogalacturonan lyase, rhamnogalacturonan acetyl esterase, xylogalacturonosidase, xylogalacturonase, rhamnogalacturonan lyase, lignin peroxidase, manganese-dependent peroxidase, hybrid peroxidase, and laccase.
18 . The method of claim 11 , wherein the spent whole fermentation broth of the recombinant microorganism is supplemented by the addition of one or more enzymes selected from the group consisting of a cellulase, endoglucanase, cellobiohydrolase, and beta-glucosidase.
19 . The method of claim 11 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
20 . The method of claim 11 , wherein the one or more substances are selected from the group consisting of an alcohol, an organic acid, a ketone, an aldehyde, an amino acid, a gas, and a combination thereof.