Nucleic acid encoding a self-assembling split-fluorescent protein system
View Patent ↗The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.
1. An isolated polypeptide comprising beta-strands 1-10 of a fluorescent protein comprising an amino acid sequence at least 95% identical to:
the sequence set forth as SEQ ID NO: 4, wherein residue 39 is I, residue 105 is K, residue 111 is V, residue 128 is T, residue 166 is T, residue 167 is V and residue 205 is T; or
the sequence set forth as SEQ ID NO: 6, wherein residue 80 is Q, residue 99 is Y, residue 105 is N, residue 111 is V, residue 128 is T, residue 166 is T, residue 172 is V and residue 205 is T; and
wherein the beta-strands 1-10 of the fluorescent protein are capable of self-complementation with a polypeptide corresponding to beta-strand 11 of the fluorescent protein.
2. The isolated polypeptide of claim 1 , wherein the beta-strands 1-10 of the fluorescent protein comprise the amino acid sequence set forth as SEQ ID NO: 4.
3. The isolated polypeptide of claim 1 , wherein the beta-strands 1-10 of the fluorescent protein consist of the amino acid sequence set forth as SEQ ID NO: 4.
4. The isolated polypeptide of claim 1 , wherein the beta-strands 1-10 of the fluorescent protein comprise the amino acid sequence set forth as SEQ ID NO: 6.
5. The isolated polypeptide of claim 1 , wherein the beta-strands 1-10 of the fluorescent protein consist of the amino acid sequence set forth as SEQ ID NO: 6.
6. The isolated polypeptide of claim 1 , wherein the fluorescent protein is a Green Fluorescent Protein (GFP).