Biocompatible phase invertible proteinaceous compositions and methods for making and using the same
View Patent ↗Biocompatible phase invertible proteinaceous compositions and methods for making and using the same are provided. Phase invertible compositions in accordance with the invention are prepared by combining a liquid proteinaceous substrate and a liquid crosslinking composition, where the liquid crosslinking composition includes a macromolecular crosslinking agent. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.
1. A method of producing a phase invertible composition, said method comprising:
combining:
(a) a liquid proteinaceous substrate; and
(b) a liquid crosslinking composition comprising a macromolecular crosslinking agent which comprises a reaction product of an excess of a liquid aldehyde crosslinking agent and a glycosaminoglycan, wherein the liquid crosslinking composition has been formed prior to combining with the liquid proteinaceous substrate to produce said phase invertible composition;
wherein said phase invertible composition is exposed to radiation.
2. The method according to claim 1 , wherein the radiation sterilizes the phase invertible composition.
3. The method according to claim 1 , wherein the radiation is gamma radiation.
4. The method according to claim 1 , wherein the radiation is electron beam radiation.
5. The method according to claim 1 , wherein said glycosaminoglycan is hyaluronan.
6. The method according to claim 1 , wherein said proteinaceous substrate comprises a proteinaceous material selected from the group consisting of: albumin, elastin, fibrin and soluble and insoluble forms of collagen and combinations thereof.
7. The method according to claim 6 , wherein said liquid aldehyde crosslinking agent comprises glutaraldehyde.
8. The method according to claim 7 , wherein said glutaraldehyde is heat stabilized.
9. A method of producing a phase invertible composition, said method comprising:
combining:
(a) a liquid proteinaceous substrate; and
(b) a liquid crosslinking composition comprising a macromolecular crosslinking agent which comprises a reaction product of an excess of a liquid aldehyde crosslinking agent and hyaluronic acid, wherein the liquid crosslinking composition has been formed prior to combining with the liquid proteinaceous substrate to produce said phase invertible composition;
wherein said phase invertible composition is exposed to radiation.