POLYELECTROLYTE-COATED SIZE-EXCLUSION ION-EXCHANGE PARTICLES
A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.
1 . A solution comprising:
a particle, the particle comprising:
a core comprising ion-exchange material; and
a coating covering the exterior surface of the particle wherein the coating comprises polyelectrolyte material,
wherein the core and coating are adapted to separate DNA sequencing reaction products;
a non-ionic surfactant; and
at least one stabilizing additive wherein the stabilizing additive is a non-ionic compound.
2 . The solution of claim 1 , wherein the non-ionic surfactant is adapted to facilitate removal of unincorporated dye terminators from the DNA sequencing reaction.
3 . The solution of claim 2 , wherein the non-ionic surfactant is effective without heating.
4 . The solution of claim 1 , wherein the ion-exchange material is cationic.
5 . The solution of claim 4 , further comprising an anion-exchange particle.
6 . The solution of claim 1 , wherein the ion-exchange material is anionic.
7 . The solution of claim 6 , further comprising a cation-exchange particle.
8 . The solution of claim 1 , wherein the non-ionic surfactant is a zwitter-ionic surfactant.
9 . The solution of claim 8 , wherein the zwitter-ionic surfactant is CHAPS.
10 . The solution of claim 1 , wherein the at least one non-ionic stabilizing additive is a zwitter-ionic compound.
11 . The solution of claim 10 , wherein the at least one zwitter-ionic stabilizing additive compound is betaine.
12 . A method for DNA sequencing, the method comprising:
providing a plurality of particles, wherein each particle comprises a core comprising ion-exchange material and a coating covering the exterior surface of the particle wherein the coating comprises polyelectrolyte material;
contacting the plurality of particles with DNA sequencing reaction products;
providing a non-ionic detergent; and
stabilizing the solution with a zwitter-ionic compound.
13 .- 17 . (canceled)
18 . A kit for purification of DNA sequencing products, the kit comprising:
a mixture of cationic and anionic particles, wherein at least one of the cationic and anionic type of particles comprise:
a core comprising ion-exchange material; and
a coating covering the exterior surface of the particle wherein the coating comprises polyelectrolyte material, wherein the core and coating are adapted to separate DNA sequencing reaction products;
a non-ionic surfactant; and
at least one stabilizing additive wherein the stabilizing additive is a non-ionic compound.
19 . The kit of claim 18 , wherein the mixture, the surfactant, and the at least one stabilizing additive are packaged together.
20 . The kit of claim 18 , wherein the mixture, the surfactant, and the at least one stabilizing additive are packaged separately.
21 .- 24 . (canceled)
25 . The solution of claim 1 , wherein the polyelectrolyte material is not crosslinked.
26 . The solution of claim 1 , wherein the polyelectrolyte material is a synthetic polymer having at least one type of charged monomer.
27 . The solution of claim 26 , wherein the polyelectrolyte synthetic polymer is a homopolymer.
28 . The kit of claim 18 , wherein the non-ionic surfactant is a zwitter-ionic surfactant.
29 . The kit of claim 18 , wherein the at least one non-ionic stabilizing additive is a zwitter-ionic compound.
30 . The kit of claim 18 , wherein the polyelectrolyte material is not crosslinked.