Preparation of stabilized catalase enzymes using polyvinyl alcohol
View Patent ↗There is provided a method of producing a stabilized catalase enzyme. In the method, a substrate is thoroughly mixed with phosphate borate and catalase, rinsed with water and the solids dried. The dried solid may be mixed with polyvinyl alcohol and dried for further stabilization. The stabilized powder may be mixed with various skin solutions (lotions, ointments and the like). The catalase enzyme can catalyze the reaction of peroxide to oxygen.
1. A method of preparing stabilized catalase enzyme comprising the steps of mixing a substrate comprising microcrystalline cellulose powder, a buffer, and catalase enzyme having a molecular weight of less than 500,000 Daltons to create a mixture including solids and liquid, draining the liquid from the mixture, rinsing the solids with water, thoroughly mixing the solids with polyvinyl alcohol, and drying the mixture to create a powder, wherein the stabilized catalase enzyme exhibits a decreased loss in catalase activity compared to catalase enzyme not mixed with the substrate and the polyvinyl alcohol, wherein the stabilized catalase enzyme has an activity at 25° C. between 500 IU/g and 1,000,000 IU/g, and wherein the stabilized catalase enzyme is stable for up to 16 weeks at 35° C. after thermal cycling of the stabilized catalase enzyme at 40° C. for 72 hours and 55° C. for 6 hours.
2. The method of claim 1 , wherein the ratio of substrate to catalase enzyme is between 1 and 10.
3. The method of claim 1 , wherein said catalase enzyme includes manganese atoms.
4. The method of claim 1 , wherein said catalase enzyme is derived from fungus.
5. The method of claim 4 , wherein the fungus is Aspergillus niger.
6. The method of claim 1 , wherein the polyvinyl alcohol has a molecular weight between 85,000 and 124,000 Daltons.
7. The method of claim 1 , wherein the stabilized catalase enzyme can decompose from above 60% to 91% hydrogen peroxide after thermal cycling when the ratio of the stabilized catalase enzyme to hydrogen peroxide is 1:1.
8. The method of claim 1 , wherein the buffer comprises phosphate and borate.