Preparation of stabilized catalase enzymes with a surfactant
View Patent ↗There is provided a method of producing a stabilized microcrystalline cellulose powder containing catalase enzyme. In the method, cellulose is thoroughly mixed with phosphate borate and catalase, rinsed with water and a surfactant added. The stabilized powder may be mixed with various skin solutions (lotions, ointments and the like). The catalase enzyme can catalyze the reaction of peroxide to oxygen.
1. A method of preparing stabilized catalase enzyme comprising the steps of mixing a substrate comprising microcrystalline cellulose powder, a buffer, and catalase enzyme having a molecular weight of less than 500,000 Daltons to create a mixture including solids and liquid, draining the liquid from the mixture, rinsing the solids with water, and then adding a surfactant to the washed solids, wherein the stabilized catalase enzyme exhibits a decreased loss in catalase activity compared to catalase enzyme not mixed with the substrate and the surfactant, wherein the stabilized catalase enzyme has an activity at 25° C. between 500 IU/g and 1,000,000 IU/g, and wherein the stabilized catalase enzyme is stable for up to 12 weeks at 35° C. after thermal cycling of the stabilized catalase enzyme at 40° C. for 72 hours and 55° C. for 6 hours.
2. The method of claim 1 , wherein said catalase enzyme includes manganese atoms.
3. The method of claim 1 , wherein said catalase enzyme is obtained from fungus.
4. The method of claim 3 , wherein the fungus is Aspergillus niger.
5. The method of claim 1 , wherein the surfactant comprises Tween 20.
6. The method of claim 1 , wherein the stabilized catalase enzyme can decompose from 60% to 95% of hydrogen peroxide after thermal cycling when the ratio of the stabilized catalase enzyme to hydrogen peroxide is 1:1.
7. The method of claim 1 , wherein the buffer comprises phosphate and borate.