Device and methods for detecting analytes in saliva
The invention provides a device for detecting drugs of abuse or other compounds in saliva. The invention thus provides a device for detecting the presence of one or more analytes in a saliva sample, comprising: (a) One or more pre-treatment regions for specifically or non-specifically removing at least a part of the fraction of the saliva sample interfering with detection of the one or more analytes; and (b) A detection region comprising a biosensor surface, the surface comprising: molecules capable of specifically binding the one or more analytes; or the one or more analytes and/or analyte analogues.
1. A microfluidic device for detecting the presence of one or more analytes in a saliva sample, comprising:
(a) one or more pre-treatment chambers comprising a pre-treatment non-porous surface with hydroxyapatite bound as a first molecule capable of specifically or non-specifically binding to one or more saliva components in the saliva sample interfering with the detection of the one or more analytes to thereby specifically or non-specifically remove at least a part of the one or more saliva components in the saliva sample interfering with detection of the one or more analytes; and
(b) a detection chamber separate from and fluidically coupled to the one or more pre-treatment chambers and comprising a biosensor surface, the biosensor surface comprising:
second molecules capable of specifically binding the one or more analytes; or
the one or more analytes and/or second molecules which are structurally related to the analyte and bind analyte-specific probes.
2. The device according to claim 1 , the one or more pre-treatment chambers further containing a third molecule being magnetizable particles coated with said first molecule capable of specifically or nonspecifically binding to the one or more components in the saliva sample interfering with detection of the one or more analytes.
3. The device according to claim 1 , wherein the hydroxyapatite is porous.
4. The device according to claim 1 , wherein the hydroxyapatite has a sintered porous structure.
5. The device according to claim 2 , wherein the hydroxyapatite is porous.
6. The device according to claim 2 , wherein the hydroxyapatite has a sintered porous structure.
7. The device according to claim 1 , which further comprises an intermediary region for containing the saliva sample prior to contacting the sample with the detection chamber.
8. The device according to claim 2 , which further comprises an intermediary region for containing the saliva sample prior to contacting the sample with the detection chamber.
9. The device according to claim 4 , which further comprises an intermediary region for containing the saliva sample prior to contacting the sample with the detection chamber.
10. The device according to claim 1 wherein the detection chamber containing magnetizable particles coated with the second molecule capable of specifically binding the one or more analytes.
11. The device according to claim 2 , wherein the detection chamber containing magnetizable particles coated with the second molecule capable of specifically binding the one or more analytes.
12. The device according to claim 4 , wherein the detection chamber containing magnetizable particles coated with the second molecule capable of specifically binding the one or more analytes.
13. The device according to claim 1 , wherein the device is a single use device.
14. The device according to claim 1 , wherein the one or more saliva components in the saliva sample interfering with the detection of the one or more analytes are selected from the group consisting of mucins, mucopolysaccharides and glycoproteins.