Microscope apparatus for preventing unnecessary degradation of a light detector
Unnecessary degradation of a light detector is prevented. Provided is a microscope apparatus ( 100 ) including: a scanner ( 5 ) that that performs scanning of illumination light emitted from a light source ( 3 ) on a specimen in two directions intersecting each other; an objective lens ( 7 ) that collects fluorescence produced in the specimen; a dispersive element ( 15 ) that disperses the fluorescence collected by the objective lens ( 7 ) into spectral components; a multichannel detector ( 20 ) that has a plurality of cells ( 21 ) for detecting the spectral components obtained through the dispersion performed by the dispersive element ( 15 ); a grouping control section ( 31 ) that groups the plurality of cells ( 21 ) of the multichannel detector ( 20 ) into a used group and an unused group; and a sensitivity control section ( 33 ) that turns off the sensitivities of the cells that are grouped into the unused group by the grouping control section ( 31 ) or reduces the sensitivities thereof with respect to the sensitivities of the cells that are grouped into the used group.
1. A method for a microscope apparatus, the method comprising:
performing scanning of illumination light emitted from a light source on a specimen in two directions intersecting each other;
collecting fluorescence produced in the specimen;
dispersing the collected fluorescence into spectral components;
detecting the dispersed spectral components by a light detector that has a plurality of cells, wherein sensitivities of each of the plurality of cells are individually adjustable by individually controlling a voltage which is applied to each of the plurality of cells and used to adjust the sensitivities of each of the plurality of cells;
receiving input from a user for selecting at least one cell from among the plurality of cells of the light detector;
grouping the plurality of cells of the light detector into a used group and an unused group according to the at least one cell selected based on the input by the user;
by individually controlling the voltage which is applied to each of the plurality of cells, turning off sensitivities of cells that are grouped into the unused group or reducing the sensitivities thereof with respect to sensitivities of cells that are grouped into the used group;
generating a first synchronization signal that is synchronized with a timing of scanning in a first scanning direction and generating a second synchronization signal that is synchronized with a timing of scanning in a second scanning direction; and
individually controlling the sensitivities of each of the cells of the unused group by individually controlling the voltage which is applied to each of the plurality of cells, in synchronization with the first synchronization signal or the second synchronization signal.
2. A microscope apparatus comprising:
a pair of galvanometer mirrors that perform scanning of illumination light emitted from a light source on a specimen in two directions intersecting each other;
an objective lens that collects fluorescence produced in the specimen;
one of a prism and a diffraction grating that disperses the fluorescence collected by the objective lens into spectral components;
a light detector that has a plurality of cells for detecting the spectral components obtained through the dispersion performed by the one of the prism and the diffraction grating, wherein sensitivities of each of the plurality of cells are individually adjustable by individually controlling a voltage which is applied to each of the plurality of cells and used to adjust the sensitivities of each of the plurality of cells;
a graphical user interface that includes a display and that is configured to receive input from a user for selecting at least one cell from among the plurality of cells of the light detector; and
a computer that is configured to operate as:
a grouping control section that groups the plurality of cells of the light detector into a used group and an unused group according to the at least one cell selected based on the input by the user; and
a sensitivity control section that, by individually controlling the voltage which is applied to each of the plurality of cells, turns off sensitivities of cells that are grouped into the unused group by the grouping control section or reduces the sensitivities thereof with respect to sensitivities of cells that are grouped into the used group,
wherein the microscope apparatus further comprises a signal output section for sending, to the sensitivity control section, a first synchronization signal that is synchronized with a timing of scanning by the pair of galvanometer mirrors in a first scanning direction and a second synchronization signal that is synchronized with a timing of scanning by the pair of galvanometer mirrors in a second scanning direction, and
wherein the sensitivity control section individually controls the sensitivities of each of the cells of the unused group by individually controlling the voltage which is applied to each of the plurality of cells, in synchronization with the first synchronization signal or the second synchronization signal, which is sent by the signal output section.