Bacterial challenge model in cattle using a trans- and intra-dermal route to infect peripheral lymph nodes

1. A method of observing and evaluating bacterial infections within the lymph nodes of animals presented for harvest comprising:

inoculating intradermally with a device capable of inoculating concurrently at multiple sites an animal with a known amount of a bacterial pathogen, wherein the multiple inoculation sites comprise lymph node drainage areas, wherein the bacterial pathogen that is a live bacterial pathogen selected from Salmonella, Listeria, Shigella, Fransicella, Clostridum, Staphylococcus, Streptococcus , or Bacillus ; and

at one or more time points obtaining one or more lymph node biopsies are aseptically harvested to determine the extent of the bacterial pathogen in the lymph nodes.

2. The method of claim 1 , wherein the inoculation is subdermal.

3. The method of claim 1 , wherein the lymph node drainage areas comprise at least one of subiliac, popliteal, retropharangeal, superficial cervical, and mesenteric.

4. The method of claim 1 , wherein the animals comprise bovine, equine, ovine, porcine, or caprine.

5. The method of claim 1 , wherein the bacterial pathogen is selected from Salmonella Newport and Montevideo.

6. A method of observing and evaluating bacterial infections within the lymph nodes of animals presented for harvest comprising:

inoculating intradermally with a multi inoculator at one or more sites of an animal a known amount of a bacterial pathogen, wherein the one or more inoculation sites comprise lymph node drainage areas, wherein the bacterial pathogen is a live bacterial pathogen selected from Salmonella, Listeria, Shigella, Fransicella, Clostridum, Staphylococcus, Streptococcus , or Bacillus;

treating the animal with one or more therapies, treatments, or exposure;

at one or more time points obtaining one or more lymph node biopsies are aseptically harvested to determine the extent of the bacterial pathogen in the lymph nodes; and

determining if the one or more therapies, treatments, or exposure were effective to eliminate or reduce the bacterial pathogen.

7. The method of claim 6 , wherein the inoculation is subdermal.

8. The method of claim 6 , wherein the lymph node drainage areas comprise at least one of subiliac, popliteal, retropharangeal, superficial cervical, and mesenteric.

9. The method of claim 6 , wherein the animals comprise bovine, equine, ovine, porcine, or caprine.

10. The method of claim 6 , wherein the bacterial pathogen is selected from Salmonella Newport and Montevideo.

11. A method of testing a compound for elimination of bacterial infections within the lymph nodes of animals presented for harvest comprising:

inoculating intradermally with a multi inoculator at one or more sites of an animal a known amount of a bacterial pathogen, wherein the one or more inoculation sites comprise lymph node drainage areas, wherein the bacterial pathogen that is a live bacterial pathogen selected from Salmonella, Listeria, Shigella, Fransicella, Clostridum, Staphylococcus, Streptococcus , or Bacillus;

treating the animal with one or more compounds;

at one or more time points obtaining one or more lymph node biopsies are aseptically harvested to determine the extent of the bacterial pathogen in the lymph nodes; and

determining if the compound was effective to eliminate or reduce the bacterial pathogen.

12. The method of claim 11 , wherein the inoculation is subdermal.

13. The method of claim 11 , wherein the lymph node drainage areas comprise at least one of subiliac, popliteal, retropharangeal, superficial cervical, and mesenteric.

14. The method of claim 11 , wherein the animals comprise bovine, equine, ovine, porcine, or caprine.

15. The method of claim 11 , wherein the bacterial pathogen is selected from Salmonella Newport and Montevideo.