System for portable and easy-to-use detection of analytes with mobile computing device
This system takes in raw cellular material collected using a provided swab, blood collection device, urine collection device, or other sample collection device and transforms that biological material into a digital result, identifying the presence, absence and/or quantity of nucleic acids, proteins, and/or other molecules of interest.
1. A system for identifying the presence, absence, and/or quantity of a molecule of interest in a sample, comprising:
a reader; and
a cartridge that fits into the reader, the cartridge configured to accept a sample collection device that fits into the cartridge,
wherein the cartridge comprises:
a first reservoir for storing a liquid, wherein a mixture comprising the liquid and the sample is formed in the first reservoir when the sample collected by the sample collection device is inserted into the first reservoir, and wherein the mixture further comprises a plurality of magnetic particle complexes;
a second reservoir for storing a chemical substrate;
an analysis channel comprising an electrochemical sensor; and
valve-actuating elements for transferring the mixture from the reservoir directly into the analysis channel and the chemical substrate from the second reservoir directly into the analysis channel, wherein the mixture localized over the electrochemical sensor reacts with the chemical substrate at the electrochemical sensor in the analysis channel for detection by the electrochemical sensor, and
wherein the reader comprises a permanent magnet adjacent to the analysis channel when the cartridge fits into the reader such that the plurality of magnetic particle complexes can be localized in the analysis channel at the electrochemical sensor within a magnetic field generated by the permanent magnet.
2. The system of claim 1 , wherein each of the plurality of magnetic particle complexes comprises a magnetic particle.
3. The system of claim 1 , wherein the permanent magnet comprises more than one magnet.
4. The system of claim 1 , wherein the electrochemical sensor releases a flow of electrons in a quantity proportional to a number of the plurality of magnetic particle complexes localized at the electrochemical sensor in the analysis channel in response to reactions between the mixture and the chemical substrate.
5. The system of claim 1 , wherein the molecule of interest comprises one or more of a nucleic acid, protein, or small molecule.
6. The system of claim 1 , wherein the reaction detected by the electrochemical sensor is proportional to a number of signaling enzymes of the plurality of magnetic particle complexes localized over the electrochemical sensor in the analysis channel.
7. The system of claim 6 , wherein the second reservoir is configured to store a liquid comprising the chemical substrate, and wherein the chemical substrate is configured to react with the signaling enzymes.
8. The system of claim 1 , wherein the cartridge comprises a slot to allow for the permanent magnet to slide into place underneath the electrochemical sensor.
9. The system of claim 1 , wherein the reader is configured to send or receive, or both, signals to a mobile device indicative of a test result.
10. The system of claim 9 , further comprising an application stored on the mobile device, the application configured to cause the mobile device to display the test result.
11. The system of claim 1 , wherein the reader is configured to send or receive, or both, signals to a mobile device indicative of a test protocol.
12. The system of claim 1 , wherein the cartridge further comprises a first valve between the first reservoir and the analysis channel and a second valve between the second reservoir and the analysis channel.
13. The system of claim 12 , wherein the first and second valves are phase change valves.
14. The system of claim 13 , wherein the valve-actuating elements comprise vias configured to heat the phase change valves to transfer the mixture and the chemical substrate to the analysis channel.
15. The system of claim 1 , further comprising a sonicator configured to direct energy into the mixture in the first reservoir.
16. The system of claim 1 , further comprising a third reservoir for storing a wash solution, wherein one of the valve-actuating elements facilitates transfer of the wash solution to the analysis channel.
17. The system of claim 1 , wherein the system further comprises the sample collection device, the sample collection device configured to collect the sample from blood, urine, or saliva.