Porcine reproductive and respiratory syndrome virus, compositions, vaccine and methods of use
The present invention relates to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) mutant strains having increased stability; compositions containing the strains or antigens derived therefrom; vaccines containing the strains, including killed, attenuated or subunit vaccines; nucleic acids encoding PRRSV polypeptides, polypeptides encoded by the nucleic acids, including antigenic fragments thereof; antibodies which specifically bind to said polypeptides, and methods making and using thereof.
1. An immunogenic composition of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), comprising at least one of
a) a composition comprising SEQ ID NO:2 (attenuated Wetzel p41); or
b) a polypeptide selected from the group consisting of
i) SEQ ID NO:9 (M protein of Wetzel p3 and of Wetzel p41),
ii) SEQ ID NO:10 (N protein of Wetzel p3and of Wetzel p41),
iii) SEQ ID NO:11 (ORF1a protein of Wetzel p41),
iv) SEQ ID NO:12 ORF1b protein of Wetzel p41),
v) SEQ ID NO:13 (GP2 protein of Wetzel p41),
vi) SEQ ID NO:14 (GP3 protein of Wetzel p41), and
vii) SEQ ID NO:15 (GP5 protein of Wetzel p41).
2. An isolated Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), comprising
a) SEQ ID NO:2, or
b) a nucleic acid sequence 97% identical to SEQ ID NO:2, which encodes a polypeptide selected from the group consisting of SEQ ID NO: 11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15.
3. The PRRSV of claim 2 , comprising an attenuated, non-virulent form of a PRRSV.
4. A vaccine for generating an immune response by a virulent PRRSV, comprising a killed or attenuated form of a PRRSV of claim 2 .
5. A method of generating an immune response in a mammal, comprising administering an immunologically-effective amount of a PRRSV of claim 2 .
6. A method of generating an immune response in a mammal, comprising administering an immunologically-effective amount of a vaccine of claim 4 .
7. The method of claim 6 , wherein the mammal is a swine.
8. The method of claim 6 , wherein the vaccine provides protective immunity to a disease caused by PRRSV infection.