COMPOSITION AND METHOD OF PREPARATION OF BONE ALLOGRAFT FROM ENDOSTEAL PORTION OF BONE AND ISOLATED BONE PERIOSTEUM
Particulate bone and structural bone, more specifically decalcified bone from endosteum having osteoinductive capacity and methods for their isolation and production are provided, more specifically, decalcified bone preparations derived from endosteal layer of bone are provided. Compositions formulated with material from endosteal layer of bone are provided along with preparation and methods of using decalcified endosteal compositions are disclosed. Endosteum is avascular and areolar in distinction to bone marrow which fills the canal. Because of its structure and anatomic position, endosteum is difficult to isolate and recognize, and therefore not regarded as an important constituent of allograft materials. One embodiment of the present invention allows for recognition and isolation of endosteum.
1 . A bone repair composition comprising particulate composition or membranous composition of cells, the particulate composition having a higher percentage of particles from an endosteum cellular layer or endosteal bone.
2 . Bone repair composition of claim 1 wherein particulate composition is preserved by freeze-drying.
3 . Bone repair composition of claim 1 wherein said composition is preserved by freeze-drying.
4 . Bone repair composition of claim 1 wherein said composition is preserved by hypothermic dehydration.
5 . Bone repair composition of claim 1 wherein said composition comprises demineralized bone.
6 . Bone repair composition of claim 1 wherein said composition is partially decalcified.
7 . Bone repair composition of claim 1 wherein said composition is surface decalcified.
8 . Bone repair composition of claim 1 wherein said composition is decalcified by citric acid, ethylenediamine, tetraacetic acid, hydrochloric acid, electrolytic process or a combination thereof.
9 . Bone repair composition of claim 1 wherein said particles measure 1 to 1000 microns.
10 . Bone repair composition of claim 1 wherein endosteal cells are dispersed and separated from the trabecular bone.
11 . Bone repair composition of claim 1 wherein endosteal cells are preserved by cryopreservation, freezing, freeze-drying, hypothermic dehydration or chemical preservation.
12 . Bone repair composition of claim 1 wherein segregated endosteal cells are intermixed with particulate bone or are seeded on structural bone grafts.
13 . Bone repair composition of claim 1 wherein bone particles or cells are suspended in a solution of hydroxyethyl starch in a form of jelly, liquid, putty or paste.
14 . Bone repair composition of claim 1 wherein bone particles or cells are suspended in human or animal collagen solution comprising a liquid, jelly, putty or paste.
15 . Bone repair composition of claim 1 wherein bone particles or cells are suspended in a solution of sucrose comprising a liquid, jelly, paste or putty.
16 . Bone repair composition of claim 1 wherein the particles or cells are mixed with a group of liquids consisting of physiological salt solution, lactated Ringer's solution, any of the accepted balanced salt solutions and or blood products.
17 . Bone repair composition of claim 1 wherein separating endosteum from long bone cortex occurs without resorting to milling which includes cortical bone.
18 . Bone repair composition of claim 1 wherein endosteum is separated from long bone under aseptic conditions and processed aseptically.
19 . Bone repair composition of claim 1 wherein endosteum is identified and separated from freeze-dried bone by the action of citric acid, ethylenediamine tetraacetic acid, hydrochloric acid, electrolytic demineralization or a combination thereof.
20 . A bone repair isolated periosteal tissue composition comprising particulate composition or membranous composition, the particulate composition having a higher percentage of particles from a periosteum cellular layer or periosteal bone.
21 . Bone repair composition of claim 20 wherein periosteal particles or membranes are prepared from the periosteum alone, not including subperiosteal cortical bone.
22 . Bone repair composition of claim 21 wherein particulate composition is preserved by freeze-drying.
23 . Bone repair composition of claim 21 wherein said composition is preserved by freeze-drying.
24 . Bone repair composition of claim 23 wherein said composition is preserved by hypothermic dehydration.
25 . Bone repair composition of claim 20 wherein the composition is prepared from periosteum subjected to hypothermic dehydration.
26 . Bone repair composition of claim 20 wherein said composition comprises demineralized bone.
27 . Bone repair composition of claim 20 wherein said composition is partially decalcified.
28 . Bone repair composition of claim 20 wherein said composition is surface decalcified.
29 . Bone repair composition of claim 20 wherein said composition is decalcified by citric acid, ethylenediamine, tetraacetic acid, hydrochloric acid, electrolytic process or a combination thereof.
30 . Bone repair composition of claim 20 wherein said particles measure 1 to 1000 microns.
31 . Bone repair composition of claim 20 wherein periosteal cells are dispersed and separated from the trabecular bone.
32 . Bone repair composition of claim 20 wherein periosteal cells are preserved by cryopreservation, freezing, freeze-drying, hypothermic dehydration or chemical preservation.
33 . Bone repair composition of claim 20 wherein segregated periosteal cells are intermixed with particulate bone or are seeded on structural bone grafts.
34 . Bone repair composition of claim 20 wherein bone particles or cells are suspended in a solution of hydroxyethyl starch in a form of jelly, liquid, putty or paste.
35 . Bone repair composition of claim 20 wherein bone particles or cells are suspended in human or animal collagen solution comprising a liquid, jelly, putty or paste.
36 . Bone repair composition of claim 20 wherein bone particles or cells are suspended in a solution of sucrose comprising a liquid, jelly, paste or putty.
37 . Bone repair composition of claim 20 wherein the particles or cells are mixed with a group of liquids consisting of physiological salt solution, lactated Ringer's solution, any of the accepted balanced salt solutions and or blood products.
38 . Bone repair composition of claim 20 wherein separating periosteum from long bone cortex occurs without resorting to milling which includes cortical bone.
39 . Bone repair composition of claim 20 wherein periosteum is separated from long bone under aseptic conditions and processed aseptically.
40 . Bone repair composition of claim 20 wherein periosteum is identified and separated from freeze-dried bone by the action of citric acid, ethylenediamine tetraacetic acid, hydrochloric acid, electrolytic demineralization or a combination thereof.
41 . Bone repair composition of claim 20 wherein periosteum is prepared as a fluff composition of branching strands 400 to 2000 microns in length.