Biosensor structures for improved point of care testing and methods of manufacture thereof
The present invention relates to analytical testing devices and methods for fabricating electrochemical creatinine biosensors, and in particular using point of care electrochemical biosensors for testing for creatinine in samples. For example, the present invention may be directed to a biosensor having an electrode, a first printed layer formed on the electrode and having a first matrix that includes creatinine amidohydrolase (CNH), creatine amidinohydrolase (CRH), and sarcosine oxidase (SOX), and second printed layer formed over the first printed layer and having a second matrix that includes CRH, SOX, and catalase.
1. A biosensor comprising:
an electrode;
a first printed layer formed on the electrode comprising a first matrix that includes creatinine amidohydrolase (CNH), creatine amidinohydrolase (CRH), and sarcosine oxidase (SOX);
a second printed layer formed over the first printed layer comprising a second matrix that includes CRH, SOX, and catalase; and
a third printed layer formed over the first printed layer such that the third printed layer is disposed between the first printed layer and the second printed layer, the third printed layer comprising a third matrix that includes CRH,
wherein the second printed layer completely covers the third printed layer; and
wherein the third printed layer completely covers the first printed layer.
2. The biosensor of claim 1 , further comprising a fourth printed layer formed on the second printed layer comprising a fourth matrix that includes CRH, SOX, and catalase.
3. The biosensor of claim 1 , further comprising a silane layer formed on the electrode such that the silane layer is disposed between the electrode and the first printed layer.
4. The biosensor of claim 1 , further comprising a gamma amino silane layer formed on the electrode such that the gamma amino silane layer is disposed between the electrode and the first printed layer.
5. The biosensor of claim 1 , wherein a diameter of the electrode is less than a diameter of the first printed layer and a diameter of the second printed layer.
6. The biosensor of claim 1 , wherein the diameter of the first printed layer is less than or equal to the diameter of the second printed layer.
7. The biosensor of claim 1 , wherein the first matrix and the second matrix are polymer matrixes.
8. The biosensor of claim 1 , wherein the first matrix and the second matrix are selected from the group consisting of: polyvinyl alcohol, gelatin, acrylamide, polyethyleneglycol diacrylate, or combinations thereof.
9. The biosensor of claim 1 , wherein the first matrix and the second matrix are photoformable.
10. The biosensor of claim 1 , wherein the first matrix and the second matrix comprise a photoinitiator.
11. The biosensor of claim 10 , wherein the photoinitiator is stilbazonium or dichromate.
12. The biosensor of claim 1 , wherein the first printed layer and the second printed layer comprise a substantially concave shape.
13. The biosensor of claim 1 , wherein the first printed layer and the second printed layer comprise a substantially convex shape.