DIAGNOSIS OF MELANOMA BY NUCLEIC ACID ANALYSIS
The present invention provides methods for diagnosing melanoma and/or solar lentigo in a subject by analyzing nucleic acid molecules obtained from the subject. The present invention also provides methods for distinguishing melanoma from solar lentigo and/or dysplastic nevi and/or normal pigmented skin. The methods include analyzing expression or mutations in epidermal samples, of one or more skin markers. The methods can include the use of a microarray to analyze gene or protein profiles from a sample.
1 . A method for diagnosing melanoma in a subject, comprising:
(a) detecting nucleic acids expressed by two or more genes comprising: ACTN4, LINC00518, CMIP, TTC3, TMEM80, HLA-C, or PRAME in a sample of a skin lesion from the subject; and
(b) characterizing the skin lesion as melanoma, based on the detection of the nucleic acids, thereby diagnosing the presence or absence of melanoma.
2 . The method of claim 1 , provided that the detecting comprises determining the level of expression of the nucleic acids.
3 . The method of claim 1 , provided that at least one of the two or more genes is LINC00518.
4 . The method of claim 1 , provided that the two or more genes consist of LINC00518 and CMIP.
5 . The method of claim 4 , provided that at least one nucleic acid comprises RNA.
6 . The method of claim 1 , further comprising amplifying nucleic acid obtained from the sample prior to detecting.
7 . The method of claim 1 , provided that the sample is obtained by applying an adhesive tape to a target area of skin in a manner sufficient to isolate the sample adhering to the adhesive tape.
8 . The method of claim 7 , provided that about one to ten adhesive tapes or one to ten applications of a tape are applied and removed from the skin.
9 . The method of claim 7 , provided that the tape comprises a rubber adhesive on a polyurethane film.
10 . The method of claim 1 , further comprising administering a treatment regimen based on the diagnosis.
11 . The method of claim 1 , provided that nucleic acid, or an amplification product thereof, is applied to a microarray.
12 . The method of claim 1 , provided that an expression profile is detected using a microarray.
13 . The method of claim 1 , provided that the sample is obtained from a biopsy sample taken at the site of the skin lesion or surrounding margin.
14 . A method of distinguishing melanoma from dysplastic nevi or normal pigmented skin in a subject, comprising:
(a) detecting nucleic acids expressed by two or more genes comprising: ACTN4, LINC00518, CMIP, TTC3, TMEM80, HLA-C, or PRAME in a sample from the subject; and
(b) characterizing the skin sample as melanoma, dysplastic nevi or normal pigmented skin, based on the detection of the nucleic acids, thereby distinguishing melanoma from dysplastic nevi or normal pigmented skin in a subject.
15 . The method of claim 14 , provided that detecting comprises determining the level of expression of the nucleic acids.
16 . The method of claim 14 , provided that at least one of the two or more genes is LINC00518.
17 . The method of claim 14 , provided that the two or more genes comprise LINC00518 and CMIP.
18 . The method of claim 14 , provided that the sample is obtained by applying an adhesive tape to a target area of skin in a manner sufficient to isolate the sample adhering to the adhesive tape.
19 . A system for diagnosing melanoma in a subject, comprising:
(a) a computer processing device, optionally connected to a computer network; and
(b) a software module executed by the computer processing device to analyze nucleic acids from two or more genes comprising: ACTN4, LINC00518, CMIP, TTC3, TMEM80, HLA-C, or PRAME in a sample from a skin lesion, to a standard or control.
20 . The system of claim 19 , wherein LINC00518 and CMIP are analyzed by the system.