MODIFIED DOCETAXEL LIPOSOME FORMULATIONS
The present invention provides compositions for the treatment of cancer. The compositions include liposomes containing a phosphatidylcholine lipid, a sterol, a PEG-lipid, and a taxane. The PEG-lipid constitutes from about 2 to about 8 mol % of the lipids in the liposome. The taxane is docetaxel esterified at the 2′-O position with a heterocyclyl-(C 2-5 alkanoic acid). Methods for preparation of liposomal taxanes and treatment of cancer with liposomal taxanes are also disclosed.
1 . A method for preparing a liposomal taxane, the method comprising:
a) forming a first liposome having a lipid bilayer comprising a phosphatidylcholine lipid and a sterol, wherein the lipid bilayer encapsulates an interior compartment comprising an aqueous solution;
b) loading the first liposome with a taxane, or a pharmaceutically acceptable salt thereof, to form a loaded liposome, wherein the taxane is docetaxel esterified at the 2′-O-position with a heterocyclyl-(C 2-5 alkanoyl) group; and
c) forming a mixture comprising the loaded liposome and a poly(ethylene glycol)-phospholipid conjugate (PEG-lipid) under conditions sufficient to allow insertion of the PEG-lipid into the lipid bilayer;
thereby forming the liposomal taxane.
2 . The method of claim 1 , wherein the liposomal taxane has a drug to lipid ratio of from 0.12 to 0.25.
3 . The method of claim 1 , wherein the liposomal taxane has a drug to lipid ratio of from 0.14 to 0.19.
4 . The method of claim 1 , wherein the sterol present in the liposomal taxane is cholesterol, and is present in an amount of about 30% to 45% by weight relative to the amount of lipids.
5 . The method of claim 1 , wherein the sterol present in the liposomal taxane is cholesterol, and is present in an amount of about 40% to 45% by weight relative to the amount of lipids.
6 . The method of claim 1 , wherein the first liposome is formed from a lipid cholesterol combination selected from the group consisting of DSPC/DSPE/Chol, 45/10/45; DOPC/Chol, 55/45; DOPC/Chol, 65/35; HSPC/Chol, 55/45; DSPC/Chol, 55/45; DMPC/Chol, 55/45; DSPC/Chol, 65/35; DPPC/Chol, 55/45; SOPC/Chol, 55/45; POPC/Chol, 55/45; HSPC/Chol, 65/35; and wherein insertion of said PEG-lipid results in an amount of PEG-lipid of from about 1.9% to about 5.0% by weight relative to the combined abouts of lipid, cholesterol and PEG-lipid.
7 . The method of claim 1 , wherein the first liposome is formed from a lipid cholesterol combination selected from the group consisting of SOPC/Chol and POPC/Chol, wherein cholesterol is present in an amount of about 42-48 mol %, and wherein insertion of said PEG-lipid results in an amount of PEG-lipid of from about 1.9% to about 5.0% by weight relative to the combined amounts of lipid, cholesterol and PEG-lipid.
8 . The method of claim 1 , wherein the first liposome is formed from a lipid cholesterol combination selected from the group consisting of DOPC/Chol, HSPC/Chol, DSPC/Chol, and DPPC/Chol, wherein cholesterol is present in an amount of about 30-48 mol %, and wherein insertion of said PEG-lipid results in an amount of PEG-lipid of from about 1.9% to about 5.0% by weight relative to the combined abouts of lipid, cholesterol and PEG-lipid.
9 . The method of claim 1 , wherein the heterocyclyl-(C 2-5 alkanoyl) group is selected from the group consisting of 5-(4-methylpiperazin-1-yl)-pentanoyl, 4-(4-methylpiperazin-1-yl)-butanoyl, 3-(4-methylpiperazin-1-yl)-propionoyl, 2-(4-methylpiperazin-1-yl)-ethanoyl, 5-morpholino-pentanoyl, 4-morpholino-butanoyl, 3-morpholino-propionoyl, 2-morpholino-ethanoyl, 5-(piperidin-1-yl)pentanoyl, 4-(piperidin-1-yl)butanoyl, 3-(piperidin-1-yl)propionoyl, and 2-(piperidin-1-yl)-ethanoyl.
10 . The method of claim 1 , wherein the heterocyclyl-(C 2-5 alkanoyl) group is 4-(4-methylpiperazin-1-yl)-butanoyl.
11 . The method of claim 1 , wherein the phosphatidylcholine lipid is selected from the group consisting of dipalmitoylphosphatidylcholine (DPPC), distearoylphosphatidylcholine (DSPC), hydrogenated soy phosphatidylcholine (HSPC), and mixtures thereof; and wherein the sterol is cholesterol.
12 . The method of claim 11 , wherein the lipid bilayer comprises DSPC and cholesterol, and wherein the DSPC:cholesterol ratio is about 55:45 (mol:mol).
13 . The method of claim 11 , wherein the lipid bilayer comprises DSPC and cholesterol, and wherein the DSPC:cholesterol ratio is about 70:30 (mol:mol).
14 . The method of claim 1 , wherein the interior compartment of the first liposome comprises aqueous ammonium sulfate.
15 . The method of claim 14 , wherein loading the first liposome comprises forming an aqueous solution comprising the first liposome and the taxane, or a pharmaceutically acceptable salt thereof, under conditions sufficient to allow accumulation of the taxane in the interior compartment of the first liposome.
16 . The method of claim 15 , wherein step b) is conducted at a temperature of from about 50° C. to about 70° C.
17 . The method of claim 15 , wherein step b) is conducted such that the ratio of the combined weight of the phosphatidylcholine and the sterol to the weight of the taxane is about 1:0.01 to about 1:1.
18 . The method of claim 17 , wherein the ratio of the combined weight of the phosphatidylcholine and the sterol to the weight of the taxane is about 1:0.2.
19 . The method of claim 1 , wherein the PEG-lipid is a diacyl-phosphatidylethanolamine-N-[methoxy(polyethene glycol)].
20 . The method of claim 19 , wherein the PEG-lipid is selected from the group consisting of distearoyl-phosphatidylethanolamine-N-[methoxy(polyethene glycol)-2000] (DSPE-PEG2000) and distearoyl-phosphatidylethanolamine-N-[methoxy(polyethene glycol)-5000] (DSPE-PEG5000).
21 . The method of claim 1 wherein step c) is conducted such that the ratio of the combined phosphatidylcholine and sterol to the PEG-lipid is from about 1000:1 (mol:mol) to about 20:1 (mol:mol).
22 . The method of claim 21 , wherein the ratio of the combined phosphatidylcholine and sterol to the PEG-lipid is from about 35:1 (mol:mol) to about 25:1 (mol:mol).
23 . The method of claim 21 , wherein the ratio of the combined phosphatidylcholine and sterol to the PEG-lipid is about 33:1 (mol:mol).
24 . The method of claim 21 , wherein the ratio of the combined phosphatidylcholine and sterol to the PEG-lipid is about 27:1 (mol:mol).
25 . The method of claim 1 , wherein step c) is conducted at a temperature of from about 35° C. to about 70° C.
26 . The method of claim 25 , wherein step c) is conducted at a temperature of from about 50° C. to about 55° C.
27 . The method of claim 1 , further comprising exchanging the liposomal taxane from the mixture in step c) to an aqueous solution that is substantially free of unencapsulated taxane and uninserted PEG-lipid.
28 . The method of claim 1 , further comprising lyophilizing the liposomal taxane.
29 . A liposomal taxane prepared according to the method of claim 1 .
30 . A method for treating cancer, the method comprising administering to a subject in need thereof a liposomal taxane prepared according to the method of claim 1 .
31 - 40 . (canceled)