IP Library › Granted Patent US 9,045,758
Granted Patent B2
US 9,045,758 · App. 14/209,634 · Granted Jun 2, 2015

Use of clostridial methyltransferases for generating novel strains

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Quick Facts
Patent No.
US 9,045,758
App. No.
14/209,634
Granted
Jun 2, 2015
Kind
B2
Abstract

This invention provides isolated polynucleotides encoding DNA Type I methyltransferase and uses thereof for improving transformation efficiencies of exogenous and endogenous plasmid DNA into Clostridial hosts.

Claims (9)

1. A method of generating a bacterial recombinant cell expressing a DNA Type I methyltransferase having biological activities, comprising:

(a) introducing a methylation vector into a bacterial host cell wherein said methylation vector expresses a DNA Type I methyltransferase polynucleotide; and

(b) introducing a transforming vector into the bacterial host cell wherein said transforming vector comprises plasmid DNA that is methylated by said methylation vector, wherein the methylation vector is pCOSMTI-1.

2. A method of generating a bacterial recombinant cell expressing a DNA Type I methyltransferase having biological activities, comprising:

(a) introducing a methylation vector into a bacterial host cell wherein said methylation vector expresses a DNA Type I methyltransferase polynucleotide; and

(b) introducing a transforming vector into the bacterial host cell wherein said transforming vector comprises plasmid DNA that is methylated by said methylation vector, wherein the methylation vector is pCOSMTI-2.

3. A method of generating a bacterial recombinant cell expressing a DNA Type I methyltransferase having biological activities, comprising:

(a) introducing a methylation vector into a bacterial host cell wherein said methylation vector expresses a DNA Type I methyltransferase polynucleotide; and

(b) introducing a transforming vector into the bacterial host cell wherein said transforming vector comprises plasmid DNA that is methylated by said methylation vector, wherein the methylation vector is pCOSMTI-12.

Assignments (5)
CORRECTIVE ASSIGNMENT TO CORRECT THE ASSIGNOR PREVIOUSLY RECORDED ON REEL 038709 FRAME 0722. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Recorded Oct 30, 2017
From: COSKATA, LLC
To: SYNATA BIO, INC.
Reel/Frame 044310/0659 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 16, 2016
From: COSKATA, INC.
To: SYNATA BIO, INC.
Reel/Frame 038709/0722 →
RELEASE OF SECURITY INTEREST Recorded Nov 23, 2015
From: VINOD KHOSLA AND NEERU KHOSLA, TRUSTEES OF THE KFT TRUST DATED NOVEMBER 17, 1986; KHOSLA VENTURES III, LP; ADVANCED TECHNOLOGY VENTURES VII, L.P.; ADVANCED TECHNOLOGY VENTURES VII (B), L.P.; ADVANCED TECHNOLOGY VENTURES VII (C), L.P.; ATV ENTREPRENEURS VII, LP
To: COSKATA, INC.
Reel/Frame 037153/0659 →
SECURITY INTEREST Recorded Oct 23, 2015
From: COSKATA, INC.
To: VINOD KHOSLA AND NEERU KHOSLA, TRUSTEES OF THE KFT TRUST DATED NOVEMBER 17, 1986; KHOSLA VENTURES III, LP; ADVANCED TECHNOLOGY VENTURES VII, L.P.; ADVANCED TECHNOLOGY VENTURES VII (B), L.P.; ADVANCED TECHNOLOGY VENTURES VII (C), L.P.; ATV ENTREPRENEURS VII, LP
Reel/Frame 036933/0596 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 27, 2015
From: REEVES, ANDREW
To: COSKATA, INC.
Reel/Frame 035501/0684 →