Multi-well tray and rack therefor
System, apparatuses, and methods for performing automated reagent-based analysis are provided. Also provided are methods for automated attachment of a cap to a reaction receptacle, and automated removal of a cap from a capped reaction receptacle.
1. A multi-well tray for use in an automated process comprising:
a base;
a card insert removably securable to the base;
one or more sets of wells, wherein each set comprises:
a receptacle cap well formed in the base and configured to receive a receptacle cap;
a receptacle well formed in the base and configured to receive a receptacle; and
a reagent well formed in the card insert and containing a lyophilized reagent;
a receptacle disposed in at least one of the receptacle wells; and
a cap disposed in at least one of the receptacle cap wells, wherein the cap is configured for locking attachment to the receptacle.
2. The multi-well tray of claim 1 , wherein the wells of each set of wells are disposed in alignment with each other.
3. The multi-well tray of claim 1 , wherein the reagent well of each set of wells is sealed with a frangible seal.
4. The multi-well tray of claim 1 , further comprising one or more locking arms disposed on the card insert for locking engagement with the base.
5. The multi-well tray of claim 1 , further comprising a protrusion extending from a bottom wall of the receptacle cap well of each set of wells.
6. The multi-well tray of claim 1 , further comprising a plurality of tabs protruding from a side wall of the receptacle cap well of each set of wells.
7. The multi-well tray of claim 1 , further comprising a through-hole extending from an inner surface of the receptacle well and defining an annular ledge at the perimeter of the through-hole.
8. The multi-well tray of claim 1 , further comprising a plurality of legs protruding from a side wall of the receptacle well.
9. The multi-well tray of claim 1 , wherein the reagent well is defined by a side wall and a bottom wall, and wherein the receptacle well further comprises one or more features selected from the group consisting of a plurality of bumps, a concave groove, a convex ridge, and a set of grooves and/or ridges comprising a crisscross pattern formed in a surface of the bottom wall.
10. The multi-well tray of claim 1 , wherein the reagent well further comprises a plurality of rigid guides radially protruding from a side wall thereof toward a center thereof.
11. The multi-well tray of claim 1 , wherein the reagent well further comprises one or more retention features selected from the group consisting of a tapered ring insert, a collar, and a fingered collar, wherein the one or more retention features are disposed within the well and are sized to retain the lyophilized reagent within the well.
12. The multi-well tray of claim 11 , wherein the one or more retention features define an opening therethrough that is too small to permit the lyophilized reagent to pass through the opening.
13. The multi-well tray of claim 1 , wherein the reagent well further comprises an annular ring or a screw thread formed in an inner wall thereof, wherein the annular ring or screw thread is sized to retain the lyophilized reagent within the well.
14. The multi-well tray of claim 1 , wherein the reagent well is sealed by a frangible foil.
15. The multi-well tray of claim 1 , further comprising one or more locking fingers disposed on a side surface of the base for releasably engaging a rack for holding a plurality of multi-well trays.
16. The multi-well tray of claim 1 , wherein the base and the card insert are formed from plastic.
17. The multi-well tray of claim 16 , wherein the card insert is formed of a plastic having lower permeability to moisture than the plastic forming the base.
18. The multi-well tray of claim 1 , comprising a plurality of sets of wells and wherein at least one reagent well contains a lyophilized reagent that is different from the lyophilized reagent contained in at least one other reagent well.
19. The multi-well tray of claim 1 , wherein the reagent well further comprises a capillary insert, wherein the lyophilized reagent is retained within a capillary channel of the capillary insert.
20. An assembly for use in an automated process comprising:
a rack comprising:
a chassis having a top surface and first and second opposing ends, the chassis including locking members;
a plurality of machine readable indicia including data disposed on the chassis; and
a handle disposed on the first end surface of the chassis;
two or more multi-well trays supported on the chassis of the rack, each multi-well tray comprising:
a base including locking features;
a card insert removably securable to the base; and
one or more sets of wells, wherein each set comprises:
a receptacle cap well formed in the base and configured to receive a receptacle cap;
a receptacle well formed in the base and configured to receive a receptacle; and
a reagent well formed in the card insert and containing a lyophilized reagent;
wherein the locking members of the rack are operatively engaged with the locking features of the each multi-well tray to secure the multi-well tray to the chassis of the rack;
a receptacle disposed in at least one of the receptacle wells;
a cap disposed in at least one of the receptacle cap wells, wherein the cap is configured for locking attachment to the receptacle; and
a fluid cartridge comprising:
a liquid chamber and a liquid reservoir in fluid communication with the liquid chamber, the liquid chamber and the liquid reservoir containing a reconstitution solution for reconstituting the lyophilized reagent, and
a frangible seal covering the liquid chamber and the liquid reservoir of the fluid cartridge.
21. The assembly of claim 20 , wherein the rack comprises multiple positions for receiving a tray and the locking members are disposed on opposite sides of each position and are configured to be engaged by a flexible locking finger located on opposite sides of a tray.
22. The assembly of claim 21 , including at least one machine readable indicia associated with each position for receiving a tray.
23. The assembly of claim 20 , wherein the machine readable indicia comprises identifying information regarding at least one of (1) the trays attached thereto, and (2) the cartridge rack.