UNHEATED EXTRACTION OF GENOMIC DNA IN AN AUTOMATED LABORATORY SYSTEM
A method for analyzing genomic DNA includes introducing a plurality of samples comprising human cells into individual vessels in each of a plurality of multi-vessel well plates. At least a subset of the human cells in the plurality of samples is lysed without the use of heat. DNA in the at least a subset of lysed human cells is isolated with the use of a plurality of paramagnetic beads. The isolated DNA is analyzed to identify one or more single nucleotide polymorphisms (SNPs), wherein the lysing, isolating, and analyzing steps are performed substantially in parallel for each of the plurality of samples.
1 . A method for analyzing genomic DNA, the method comprising:
introducing a plurality of samples comprising human cells into individual vessels in each of a plurality of multi-vessel well plates;
lysing at least a subset of the human cells in the plurality of samples without the use of heat;
isolating DNA in the at least a subset of lysed human cells with the use of a plurality of paramagnetic beads; and
analyzing the isolated DNA to identify one or more single nucleotide polymorphisms (SNPs), wherein the lysing, isolating, and analyzing steps are performed substantially in parallel for each of the plurality of samples.
2 . The method of claim 1 , wherein the lysing further comprises introducing one or more chemical reagents to the plurality of samples.
3 . The method of claim 2 , wherein the one or more chemical reagents comprises a chaotropic salt solution.
4 . The method of claim 2 , wherein the one or more chemical reagents comprises a protease enzyme.
5 . The method of claim 4 , wherein the protease enzyme is Proteinase K.
6 . The method of claim 1 , further comprising labeling each of the plurality of multi-vessel well plates with a barcode.
7 . The method of claim 6 , further comprising scanning the barcodes and associating the barcodes with a unique patient sample identifier in a computing device.
8 . The method of claim 7 , further comprising tracking each of the plurality of multi-vessel well plates with the computing device as the plurality of multi-vessel well plates are processed by an automated liquid handling system.
9 . The method of claim 1 , further comprising performing the introducing, lysing, isolating, and analyzing steps for at least 4000 samples in a 24 hour period.
10 . The method of claim 1 , further comprising analyzing the one or more SNPs to assess cardiovascular health, effectiveness of a cardiovascular disease treatment, or a risk of developing cardiovascular disease for a subject.
11 . The method of claim 1 , further comprising analyzing the one or more SNPs to assess diabetes, effectiveness of a diabetes treatment, or a risk of developing diabetes for a subject.
12 . The method of claim 1 , further comprising analyzing the one or more SNPs to assess fatty liver health, effectiveness of a fatty liver disease treatment, or a risk of developing fatty liver disease for a subject.
13 . The method of claim 1 , wherein the one or more SNPs are selected from the group consisting of APOE 112, APOE 158, MTHFR C677T, FII, FVL, CYP2C19*2, CYP2C19*3, CYP2C19*17, CYP2C9 *2, CYP2C9 *3 and VKORC1.
14 . The method of claim 1 , wherein the plurality of samples are selected from the group consisting of body fluids, body wastes, body excretions, and blood.
15 . The method of claim 1 , wherein the plurality of samples comprise blood.
16 . The method of claim 1 , wherein the introducing, lysing, isolating, and analyzing steps are performed at room temperature.
17 . The method of claim 1 , wherein one or more of the introducing, lysing, isolating, and analyzing steps are performed in a single liquid sample handling instrument.
18 . The method of claim 1 , wherein each of a plurality of multi-vessel well plates comprise 96-sample multi-vessel well plates.