Plant growth-promoting bacteria and methods of use
The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.
1. A method for stimulating plant growth, comprising
applying a bacterial culture to a plant, plant seed, or plant growth medium,
wherein the bacteria in the bacterial culture comprises:
(a) Bacillus aryabhattai strain CAP53 (NRRL No. B-50819);
(b) Bacillus aryabhattai strain CAP56 (NRRL No. B-50817);
(c) Bacillus flexus strain BT054 (NRRL No. B-50816);
(d) Paracoccus kondratievae strain NC35 (NRRL No. B-50820);
(e) Enterobacter cloacae strain CAP12 (NRRL No. B-50822);
(f) Bacillus nealsonii strain BOBA57 (NRRL No. B-50821); or
a mutant having all of the identifying characteristics of any one of said strains, wherein the mutant comprises one or more mutations and retains the ability to promote plant growth; and
the bacterial culture is applied in an inoculum comprising an effective amount of the bacterial culture and an agriculturally acceptable carrier.
2. The method of claim 1 wherein the inoculum comprises an effective amount of a mixture comprising at least two of the bacterial cultures of claim 1 .
3. The method of claim 1 , wherein the inoculum further comprises an effective amount of a rhizobacteria.
4. The method of claim 3 , wherein the rhizobacteria comprises Bradyrhizobium genus bacteria, Rhizobium genus bacteria, or a combination thereof.
5. The method of claim 4 , wherein the Bradyrhizobium genus bacteria comprises Bradyrhizobium japonicum.
6. The method of claim 4 , wherein the Rhizobium genus bacteria comprises Rhizobium phaseoli, Rhizobium leguminosarum , or a combination thereof.
7. The method of claim 1 , wherein the agriculturally acceptable carrier comprises a dispersant, a surfactant, an additive, water, a thickener, an anticaking agent, residue breakdown, a composting formulation, a granular application, diatomaceous earth, an oil, a coloring agent, a stabilizer, a preservative, a polymer, a coating, or a combination thereof.
8. The method of claim 1 , wherein the agriculturally acceptable carrier comprises vermiculite, charcoal, sugar factory carbonation press mud, rice husk, carboxymethyl cellulose, peat, perlite, fine sand, calcium carbonate, flour, alum, a starch, talc, polyvinyl pyrrolidone, or a combination thereof.
9. The method of claim 1 , wherein the inoculum is formulated as a seed coating formulation, a liquid formulation for application to plants or to a plant growth medium, or a solid formulation for application to the plants or to the plant growth medium.
10. The method of claim 9 , wherein the seed coating formulation is an aqueous or oil-based solution for application to seeds.
11. The method of claim 9 , wherein the seed coating formulation is a powder or granular formulation for application to seeds.
12. The method of claim 9 , wherein the liquid formulation for application to the plants or to the plant growth medium is in a concentrated formulation or a working form formulation.
13. The method of claim 9 , wherein the solid formulation for application to the plants or to the plant growth medium is a granular formulation or a powder agent.
14. The method of claim 1 , wherein the inoculum further comprises a fertilizer, a micronutrient fertilizer material, an insecticide, a herbicide, a plant growth amendment, a fungicide, a molluscicide, an algicide, a bacterial inoculant, a fungal inoculant, or a combination thereof.
15. The method of claim 14 , wherein the bacterial inoculant comprises a bacterial inoculant of the genus Rhizobium , a bacterial inoculant of the genus Bradyrhizobium , a bacterial inoculant of the genus Mesorhizobium , a bacterial inoculant of the genus Azorhizobium , a bacterial inoculant of the genus Allorhizobium , a bacterial inoculant of the genus Sinorhizobium , a bacterial inoculant of the genus Kluyvera , a bacterial inoculant of the genus Azotobacter , a bacterial inoculant of the genus Pseudomonas , a bacterial inoculant of the genus Azospirillium , a bacterial inoculant of the genus Bacillus , a bacterial inoculant of the genus Streptomyces , a bacterial inoculant of the genus Paenibacillus , a bacterial inoculant of the genus Paracoccus , a bacterial inoculant of the genus Enterobacter , a bacterial inoculant of the genus Alcaligenes , a bacterial inoculant of the genus Mycobacterium , a bacterial inoculant of the genus Trichoderma , a bacterial inoculant of the genus Gliocladium , a bacterial inoculant of the genus Glomus , a bacterial inoculant of the genus Klebsiella , or a combination thereof.
16. The method of claim 14 , wherein the insecticide comprises an organophosphate, a carbamate, a pyrethroid, an acaricide, an alkyl phthalate, boric acid, a borate, a fluoride, sulfur, a haloaromatic substituted urea, a hydrocarbon ester, a biologically-based insecticide, or a combination thereof.
17. The method of claim 14 , wherein the fungicide comprises a substituted benzene, a thiocarbamate, an ethylene bis dithiocarbamate, a thiophthalidamide, a copper compound, an organomercury compound, an organotin compound, a cadmium compound, anilazine, benomyl, cyclohexamide, dodine, etridiazole, iprodione, metlaxyl, thiamimefon, triforine, or a combination thereof.
18. The method of claim 1 , comprising applying the bacterial culture to a plant seed.
19. The method of claim 1 , wherein the bacteria in the bacterial culture is Paracoccus kondratievae strain NC35 and the bacteria has a 16S ribosomal RNA sequence having at least about 98%, at least about 99%, or 100% sequence identity with the sequence of SEQ ID NO: 4.