IP Library › Granted Patent US 9,260,527
Granted Patent B2
US 9,260,527 · App. 14/217,841 · Granted Feb 16, 2016

Anti-human CXCR4 antibodies and methods of making same

Inventors: Ross S. Chambers (Hockessin, DE); Michael C. Brown (North East, MD); Dale V. Onisk (Conowingo, MD); L. Joe Stafford (Newark, DE); Fenglin Yin (Naperville, IL)
Assignee: SDIX, LLC
C07K16/2866C12N15/09C12N15/63C07K2317/76
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 9,260,527
App. No.
14/217,841
Granted
Feb 16, 2016
Kind
B2
Abstract

The present invention provides novel antibodies to human CXCR4 with high affinity to the target and the ability to act potently as antagonists. The antibodies disclosed herein bind to a diverse range of epitopes.

Claims (31)

1. An antibody that binds CXCR4 selected from the group consisting of:

(a) an antibody comprising a first heavy chain CDR comprising SEQ ID NO: 1; a second heavy chain CDR comprising SEQ ID NO: 2; a third heavy chain CDR comprising SEQ ID NO: 3; a first light chain CDR comprising SEQ ID NO: 4; a second light chain CDR comprising SEQ ID NO: 5; and a third light chain CDR comprising SEQ ID NO: 6;

(b) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 7; a second heavy chain CDR comprising SEQ ID NO: 8; a third heavy chain CDR comprising SEQ ID NO: 9; a first light chain CDR comprising SEQ ID NO: 10; a second light chain CDR comprising SEQ ID NO: 11; and a third light chain CDR comprising SEQ ID NO: 12;

(c) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 13; a second heavy chain CDR comprising SEQ ID NO: 14; a third heavy chain CDR comprising SEQ ID NO: 15; a first light chain CDR comprising SEQ ID NO: 16; a second light chain CDR comprising SEQ ID NO: 17; and a third light chain CDR comprising SEQ ID NO: 18;

(d) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 19; a second heavy chain CDR comprising SEQ ID NO: 20; a third heavy chain CDR comprising SEQ ID NO: 21; a first light chain CDR comprising SEQ ID NO: 22; a second light chain CDR comprising SEQ ID NO: 23; and a third light chain CDR comprising SEQ ID NO: 24;

(e) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 25; a second heavy chain CDR comprising SEQ ID NO: 26; a third heavy chain CDR comprising SEQ ID NO: 27; a first light chain CDR comprising SEQ ID NO: 28; a second light chain CDR comprising SEQ ID NO: 29; and a third light chain CDR comprising SEQ ID NO: 30;

(f) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 31; a second heavy chain CDR comprising SEQ ID NO: 32; a third heavy chain CDR comprising SEQ ID NO: 33; a first light chain CDR comprising SEQ ID NO: 34; a second light chain CDR comprising SEQ ID NO: 35; and a third light chain CDR comprising SEQ ID NO: 36;

(g) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 37; a second heavy chain CDR comprising SEQ ID NO: 38; a third heavy chain CDR comprising SEQ ID NO: 39; a first light chain CDR comprising SEQ ID NO: 40; a second light chain CDR comprising SEQ ID NO: 41; and a third light chain CDR comprising SEQ ID NO: 42;

(h) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 43; a second heavy chain CDR comprising SEQ ID NO: 44; a third heavy chain CDR comprising SEQ ID NO: 45; a first light chain CDR comprising SEQ ID NO: 46; a second light chain CDR comprising SEQ ID NO: 47; and a third light chain CDR comprising SEQ ID NO: 48;

(i) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 49; a second heavy chain CDR comprising SEQ ID NO: 50; a third heavy chain CDR comprising SEQ ID NO: 51; a first light chain CDR comprising SEQ ID NO: 52; a second light chain CDR comprising SEQ ID NO: 53; and a third light chain CDR comprising SEQ ID NO: 54;

(j) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 55; a second heavy chain CDR comprising SEQ ID NO: 56; a third heavy chain CDR comprising SEQ ID NO: 57; a first light chain CDR comprising SEQ ID NO: 58; a second light chain CDR comprising SEQ ID NO: 59; and a third light chain CDR comprising SEQ ID NO: 60;

(k) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 61; a second heavy chain CDR comprising SEQ ID NO: 62; a third heavy chain CDR comprising SEQ ID NO: 63; a first light chain CDR comprising SEQ ID NO: 64; a second light chain CDR comprising SEQ ID NO: 65; and a third light chain CDR comprising SEQ ID NO: 66;

(l) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 67; a second heavy chain CDR comprising SEQ ID NO: 68; a third heavy chain CDR comprising SEQ ID NO: 69; a first light chain CDR comprising SEQ ID NO: 70; a second light chain CDR comprising SEQ ID NO: 71; and a third light chain CDR comprising SEQ ID NO: 72; and

(m) an antibody comprising: a first heavy chain CDR comprising SEQ ID NO: 73; a second heavy chain CDR comprising SEQ ID NO: 74; a third heavy chain CDR comprising SEQ ID NO: 75; a first light chain CDR comprising SEQ ID NO: 76; a second light chain CDR comprising SEQ ID NO: 77; and a third light chain CDR comprising SEQ ID NO: 78.

2. An antibody that binds CXDR4 selected from the group consisting of:

(a) an antibody comprising a heavy chain variable region comprising any one of SEQ ID NOs: 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128; or

(b) an antibody comprising a light chain variable region comprising any one of SEQ ID NOs: 82, 86, 90, 94, 98, 102, 106, 110, 114, 118, 122, 126, 130.

3. An antibody according to claim 1 , which is a humanized antibody.

4. An antibody according to claim 2 , which is a humanized antibody.

5. A protein selected from the group consisting of

(a) a protein comprising a variable heavy chain having any one of SEQ ID NOs: 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128, or

(b) a protein comprising a variable light chain having any one of SEQ ID NOs: 82, 86, 90, 94, 98, 102, 106, 110, 114, 118, 122, 126, 130.

6. A nucleic acid encoding an antibody of claim 1 .

7. A nucleic acid encoding an antibody of claim 2 .

8. A nucleic acid encoding a protein of claim 5 .

9. An expression vector which encodes an antibody of claim 1 .

10. An expression vector which encodes an antibody of claim 2 .

11. An expression vector which encodes a protein of claim 5 .

12. A method of making an antibody according to claim 1 , comprising culturing a cell comprising a nucleic acid encoding said antibody under conditions suitable for expression of said antibody.

13. A method of making an antibody according to claim 2 , comprising culturing a cell comprising a nucleic acid encoding said antibody under conditions suitable for expression of said antibody.

14. A method of making a protein according to claim 5 , comprising culturing a cell comprising a nucleic acid encoding said protein under conditions suitable for expression of said protein.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 9, 2015
From: CHAMBERS, ROSS S.; BROWN, MICHAEL C.; ONISK, DALE V.; STAFFORD, L. JOE; YIN, FENGLIN
To: SDIX, LLC
Reel/Frame 035368/0572 →
Continuity (2)
Provisional Application 61800963 · Mar 15, 2013
Related Publication 20140286936A1 · Sep 25, 2014