Device for analysis of a target analyte
This disclosure is directed to a slide for analyzing target analytes of a suspension. The slide can be used to collect and hold the target analyte for imaging or further processing. The slide comprises a well section, including wells into which the target analyte can be stored. The wells may be removable and sized to fit into a second apparatus, such as a PCR thermocycler, for additional processing. Alternatively, the wells may hold processing vessels, such as PCR tubes, the processing vessels being used for downstream processing and/or analysis.
1. A slide comprising:
a main body comprising
a viewing station to hold a sample for imaging of the sample and picking of a target analyte from the sample, and
a holding station to hold a picked target analyte prior to the picked target analyte being deposited into a processing vessel or the at least one well; and
a well section comprising at least one well,
wherein the well section is attached to the main body, and
wherein the holding station is located between the viewing station and the well section.
2. The slide of claim 1 , wherein the main body and the well section are one piece.
3. The slide of claim 1 , wherein the well section is removably attached to the main body.
4. The slide of claim 3 , wherein the well section is sized to fit into a second apparatus for further processing or analysis.
5. The slide of claim 3 , further comprising a plurality of wells, wherein at least one well of the plurality of wells is removably attached to the well section.
6. The slide of claim 1 , further comprising a wall surrounding at least a portion of the main body.
7. The slide of claim 1 , the at least one well further comprising a plug.
8. The slide of claim 1 , the holding station comprising a solution to wash, process, or label the picked target analyte.
9. The slide of claim 1 , further comprising at least one processing vessel located within at least one well.
10. The slide of claim 9 , wherein the at least one processing vessel is a PCR tube.
11. The slide of claim 1 , wherein a bottom of the viewing station, a bottom of the holding station, and a bottom of the least one well are optically clear for imaging.
12. The slide of claim 1 , further comprising:
an enclosure extending upwardly from the main body, the enclosure comprising
a first wall adjoined to a second wall and a fourth wall,
a third wall adjoined to the second wall and the fourth wall,
wherein the first and third walls opposite each other, and
wherein the second and fourth walls opposite each other; and
a partition extending from the first wall to the third wall,
wherein the viewing station includes the second wall, the partition, a first portion of the first wall and a first portion of the third wall, and
wherein the holding station includes the fourth wall, the partition, a second portion of the first wall and a second portion of the third wall.
13. The slide of claim 1 , wherein the viewing station, the holding station, and the well section extend through the main body, thereby starting at a top side of the main body and ending at a bottom side of the main body.
14. The slide of claim 1 , the holding station comprising at least one divider to form a plurality of segments within the holding station, wherein n dividers across the holding station yields n+1 segments within the holding station, and wherein n is greater than or equal to 1.
15. The slide of claim 1 , wherein the viewing station, the holding station, and the well section have the same focal plane.
16. The slide of claim 1 , wherein the viewing station and the holding station have the same focal plane as one another, and wherein the well section includes an offset such that the focal plane of the well section is adjusted from the focal plane of the viewing station and the holding station by the offset.