Method of preparing microspheres by using polymer having sol-gel transition property and microspheres prepared thereby
The present invention relates to a method of preparing microspheres by using a polymer having a sol-gel transition property and microspheres prepared thereby, and more particularly, to a method of preparing microspheres by using a polymer having a sol-gel transition property and microspheres prepared thereby capable of preventing a solvent in a polymer solution for a carrier from being rapidly diffused to the aqueous medium before formation of the microspheres to reduce porosity of the microspheres and reduce surface roughness of the microspheres in order to obtain microspheres having a sphere shape, and increasing an encapsulation ratio of a bioactive substance, by using a polymer having a sol-gel transition property as a surfactant included in an aqueous medium into which a primary emulsion is injected and gelating a secondary emulsion formed after injecting the primary emulsion using the sol-gel transition property of the polymer used as the surfactant.
1. A method of preparing microspheres, in which a bioactive substance is encapsulated in a polymer for a carrier, comprising:
1) forming a secondary emulsion by injecting a primary emulsion, in which the bioactive substance and the polymer for a carrier are mixed, into an aqueous medium in which a polymer having a sol-gel transition property is dissolved (Step 1);
2) performing a gelation reaction on the secondary emulsion (Step 2); and
3) removing a residual solvent and an aqueious medium without stirring, and collecting the microspheares after step 2 (Step 3).
2. The method of preparing microspheres of claim 1 , wherein the bioactive substance is at least one selected from the group consisting of luteinizing hormone-release hormone (LHRH) homologs, peptides, and salts thereof.
3. The method of preparing microspheres of claim 2 , wherein the bioactive substance is at least one selected from the group consisting of goserelin, leuprolide acetate, triptorelin, buserelin, nafarelin, cetrorelix, and argitide.
4. The method of preparing microspheres of claim 1 , wherein the primary emulsion is prepared by dissolving the polymer for the carrier in at least one solvent selected from the group consisting of methylene chloride, chloroform, acetonitrile, dimethylsulfoxide, dimethylformamide, and ethyl acetate.
5. The method of preparing microspheres of claim 1 , wherein the polymer for the carrier is a biodegradable polymer.
6. The method of preparing microspheres of claim 5 , wherein the polymer for the carrier is at least one selected from the group consisting of polylactide, polyglycolide, and poly(lactide-co-glycolide).
7. The method of preparing microspheres of claim 1 , wherein the polymer having the sol-gel transition property is at least one selected from the group consisting of methyl cellulose, hydroxypropylmethylcellulose (HPMC), ethylhydroxyethylcellulose (EHEC), xyloglucan, chitosan, poly(N-isopropylamide) (PNIPAM), poly(N-isopropylamide-co-acrylic acid), poloxamer (PEO-PPO-PEO), Poloxamer-g-PAA, PAA-g-poloxamer, PEO-PLGA-PEO, and poly(vinyl alcohol).
8. The method of preparing microspheres of claim 1 , wherein the removal of the residual solvent and the aqueous medium of Step 3) are performed by evaporating the residual solvent and the aqueous medium under a vacuum in the range of 0 to 0.99 Torr.
9. Microspheres comprising a bioactive substance encapsulated in the polymer for a carrier, prepared by the method of claim 1 .
10. A drug delivery system comprising the microspheres of claim 9 as an active component.
11. The drug delivery system of claim 10 , wherein the drug delivery system is in the form of an injection.