Inverted microscope system
View Patent ↗An inverted microscope system includes an objective lens holding unit that holds an objective lens configured to collect at least observation light from a specimen, a tube lens configured to form an image using the observation light collected by the objective lens, a total internal reflection fluorescence microscopy optical system provided between the objective lens and the tube lens and configured to observe the observation light from the specimen using a total reflection illumination, and a disk scanning confocal optical system including a rotary disk on which a confocal opening is formed, the confocal opening being placed at a position substantially conjugate to a focus position of the objective lens. A relative distance between the focus position of the objective lens and the substantially conjugate position is changeable along an optical path of the observation light.
1. An inverted microscope system comprising:
an objective lens holding unit that holds an objective lens configured to collect at least observation light from a specimen;
a tube lens configured to form an image using the observation light collected by the objective lens;
a total internal reflection fluorescence microscopy optical system provided between the objective lens and the tube lens and configured to observe the observation light from the specimen using a total reflection illumination; and
a disk scanning confocal optical system including a rotary disk in which a confocal opening is formed, the confocal opening being placed at a position substantially conjugate to a focus position of the objective lens,
wherein a relative distance between the focus position of the objective lens and the substantially conjugate position is changeable along an optical path of the observation light,
wherein the disk scanning confocal optical system passes through a part of an optical path of the total internal reflection fluorescence microscopy optical system,
wherein the disk scanning confocal optical system and the objective lens are disposed on opposite sides of the tube lens,
wherein the inverted microscope system further comprises at least one optical path splitting member between the tube lens and the rotary disk, and
wherein the disk scanning confocal optical system is removable from a main body that holds the objective lens holding unit and the tube lens.
2. The inverted microscope system according to claim 1 , wherein the confocal opening in the rotary disk is placed at a focus position of the tube lens.
3. The inverted microscope system according to claim 2 , wherein a movement of a position of the rotary disk along the optical path of the observation light changes the relative distance between the focus position of the objective lens and the substantially conjugate position.
4. The inverted microscope system according to claim 2 , wherein a movement of a position of the tube lens along the optical path of the observation light changes the relative distance between the focus position of the objective lens and the substantially conjugate position.
5. The inverted microscope system according to claim 2 , wherein selectively placing a plurality of permeable plane parallel boards having different thicknesses between the tube lens and the rotary disk on the optical path of the observation light changes the relative distance between the focus position of the objective lens and the substantially conjugate position.