Methods and kits for detecting mastitis
View Patent ↗Methods and kits for determining if one or more animals have mastitis and for monitoring animals and the quality of the milk they produce are disclosed. Kits and test assays disclosed are used to determine the quantity of proteasomes and proteins thereof, the activity of proteasome enzymes, the quantity of proteasome bound and regulating proteins, and the quantity of ubiquinated protein. Components and reagents for use in the kits and assays are also disclosed.
1. A method for determining proteasomal activity in milk from an animal indicative of mastitis in the animal, said method comprising
(a) contacting a test sample of milk collected from the animal with luciferase and luciferin;
(b) determining a level of adenosine triphosphate (ATP) present in the test sample by detecting hydrolysis of luciferin; and
(c) comparing the level of ATP with a standard level of ATP in a control sample of milk, wherein a lower level of ATP present in the test sample relative to the control sample is indicative of elevated proteasomal activity and mastitis in the animal.